分析化学Issue(11)：1634-1639,6.
电喷雾内部萃取电离质谱直接分析蒜瓣组织的研究
Direct Molecular Analysis of Garlic Using Internal Extractive Electrospray Ionization Mass Spectrometry
摘要
Abstract
Theapplicationofinternalextractiveelectrosprayionizationmassspectrometry(iEESI-MS)was extended to direct molecular analysis of garlic tissues. By obviating time-consuming sample preparations, fragile active garlic substances such as organosulfur compounds ( e. g. , alliin, allicin ) were successfully detected and identified via collision-induced dissociation ( CID) , together with amino acids ( e. g. , arginine) and saccharides ( glucose, polysaccharides) . Mass spectral fingerprints of different kinds of garlic cloves, as well as various post-treatment ones, were further processed via principal component analysis ( PCA) to better visualize the differences. Our experimental results indicated that iEESI-MS allowed rapid recognition of metabolic changes in the garlic tissue subject to various external stimuli. The merits included simplicity of analysis, high speed ( less than 2 min per sample ) , good specificity, and minimal disturbance to the bioactivity of analytes.关键词
电喷雾内部萃取电离/大蒜/直接分析/质谱Key words
Internal extractive electrospray ionization/Garlic/Direct analysis/Mass spectrometry引用本文复制引用
张华,朱亮,陈焕文..电喷雾内部萃取电离质谱直接分析蒜瓣组织的研究[J].分析化学,2014,(11):1634-1639,6.基金项目
长江学者和创新团队发展计划资助( No. IRT13054) （ No. IRT13054）
国家自然科学基金( No.21105010) （ No.21105010）
江西省科技厅科技计划项目(Nos.2010EHA01000,2010DD01300)资助This work was supported by the National Natural Science Foundation of China (No.21105010) （Nos.2010EHA01000,2010DD01300）
