分析化学Issue(11)：1651-1655,5.DOI:10.11895/j.issn.0253-3820.140491
待定模板印迹聚合物对高丰度鸡蛋清蛋白质的脱除
Depletion of Chicken Egg White Proteins by Pending Templates Imprinted Polymers
摘要
Abstract
Eggwhitesolutionswereusedas“pendingtemplates”topreparemolecularimprintedpolymers. The obtained polymers acted as stationary phases, exhibiting abilities to deplete abundant proteins in the egg white. The abilities of protein depletion of the imprinted polymers could be adjusted by using different concentrations of egg white in preparing the polymers. After chromatographically processed in a home-made syringe system, abundant proteins such as ovalbumin, lysozyme, and ovotransferrin were removed from egg white sample solutions. Along with the disappearance of the mass spectrum peaks of these proteins, the signals of other components became more obvious. Meanwhile according to reference data and molecular weights, they were reasonably ascribed to be ovalbumin-related proteins, ovotransferrin-related proteins, ovomucoids or flavoproteins respectively. Conclusively, pending imprinting method has the power to deplete abundant proteins, and to retain and enrich posttranslationally modified species at the same time.关键词
待定模板印迹聚合物/鸡蛋清/蛋白质脱除/富集Key words
Pendingtemplatesimprintedpolymer/Chickeneggwhite/Proteindepletion/Enrichment引用本文复制引用
杨春,栾新杰,刘国峰,周兴璐,王建,胡效亚..待定模板印迹聚合物对高丰度鸡蛋清蛋白质的脱除[J].分析化学,2014,(11):1651-1655,5.基金项目
国家自然科学基金(No.21375115)、江苏高校优势学科建设工程资助项目This work was supported by the National Natural Science Foundation of China (No.21375115) （No.21375115）
