分析化学Issue(11)：1656-1660,5.DOI:10.11895/j.issn.0253-3820.140357
电化学还原氧化石墨烯/纳米金-壳聚糖复合膜修饰玻碳电极对尿酸的灵敏测定
A Glassy Carbon Electrode Modified with Electrochemically Reduced Graphene Oxide/Gold Nanoparticles-Chitosan Composite Film for Sensitive Determination of Uric Acid
摘要
Abstract
Anelectrochemicallyreducedgrapheneoxide/goldnanoparticle-chitosan(ERGO/AuNP-CS) composite film modified glassy carbon electrode ( GCE) was constructed by directly electrochemical reduction of GO, and then assembly of AuNP-CS polycation on the surface. The surface morphologies of different modified electrodes including bare GCE, GCE/GO, GCE/ERGO and GCE/ERGO/AuNP-CS were characterized by scanning electron microscopy ( SEM ) . The differential pulse voltammetric behaviors of the electrodes were investigated, and the results indicated that the composite of ERGO/AuNP-CS exhibited excellent electrocatalytic oxidation activity to uric acid ( UA) molecule. In 0. 10 mol/L of phosphate buffer solution (pH=6. 5) with a scanning rate of 100 mV/s, the proposed composite film modified electrode showed a linear electrochemical response to UA in the range of 0 . 05-110 μmol/L with a detection limit of 12. 4 nmol/L ( S/N = 3 ). The electrode displayed good selectivity, reproducibility and stability in the determination of UA in human serum and urine samples with a recovery of 93 . 8%-104 . 1%. The detection results were agreed with those of conventional spectrophotometry and uricase Kit methods.关键词
电化学还原氧化石墨烯/纳米金-壳聚糖聚阳离子/尿酸/电催化氧化/修饰电极Key words
Electrochemicallyreducedgrapheneoxide/Goldnanoparticle-chitosanpolycation/Uricacid/Electrocatalytic oxidation/Modified electrode引用本文复制引用
吴玲,曹忠,宋天铭,宋铖,谢晶磊,何婧琳,肖忠良..电化学还原氧化石墨烯/纳米金-壳聚糖复合膜修饰玻碳电极对尿酸的灵敏测定[J].分析化学,2014,(11):1656-1660,5.基金项目
国家科技支撑计划(Nos.2012BAD31B08,2012BAC17B01)、国家自然科学基金(Nos.21275022,21105005)、湖南省科技计划(No.2013FJ3075)项目资助This work was supported by the National High Technology Research and Development Program of China ( No.2012BAD31B08,2012BAC17B01) and the National Natural Science Foundation of China (Nos.21275022,21105005) （Nos.2012BAD31B08,2012BAC17B01）
