分析化学Issue(12)：1838-1841,4.DOI:10.11895/j.issn.0253-3820.140734
在线固相萃取结合高效液相色谱-电喷雾式检测器测定鸡粪中红霉素残留
Determination of Erythrocin in Chicken Manure by High Performance Liquid Chromatography-Corona-Charged Aerosol Detection Coupled with On-line Solid Phase Extraction
摘要
Abstract
A novel HPLC-CAD method coupled with on-line solid phase extraction ( SPE ) for the determination of erythrocin which was widely used in livestock farming was developed. After mixed with diatomite, 5. 0 g manure sample was put into the cell and extracted with hot water at 70℃ and 10. 3 MPa. An on-line SPE methodology was applied to pre-treat the sample, and the sample was seperated on an Acclaim 120 C18 column and analyzed by corona CAD detector using acetonitrile and 0. 1% formic acid as mobile phase. Good linearity for erythrocin was obtained in the range of 21-2000 μg/kg. The detection limit was 6. 3 μg/kg. The average recoveries were 79. 2%-87. 5%.关键词
高效液相色谱/电喷雾式检测器/在线固相萃取/红霉素/快速溶剂萃取Key words
High performance liquid chromatography/Corona-charged aerosol detector/Online solid phase extraction/Erythrocin/Accelerated solvent extraction引用本文复制引用
周茜,陈满堂,朱丽华,丁耀彬..在线固相萃取结合高效液相色谱-电喷雾式检测器测定鸡粪中红霉素残留[J].分析化学,2014,(12):1838-1841,4.基金项目
本文系国家高技术研究发展计划(863计划)(No.2012AA06A304)资助项目This work was partially supports by the National High Technology Research and Development Program of China (No.2012AA06A304) （863计划）
