分析化学Issue(12)：1871-1872,2.DOI:10.11895/j.issn.0253-3820.140849
双三元液相色谱-串联电喷雾检测器用于中药注射剂大分子物质筛查研究
Dual-Gradient Liquid Chromatography-Tandem Charged Aerosol Detector for Detection of Macromolecules in Traditional Chinese Medicine Injections
张婷婷 1王玉 2谷丹华 3张俐 2金燕4
作者信息
- 1. 赛默飞世尔科技色谱产品应用研究中心，上海200100
- 2. 中国药科大学，南京210009
- 3. 江苏省食品药品检验所，南京210008
- 4. 江苏省食品药品检验所，南京210008
- 折叠
摘要
Abstract
A size exclusion chromatography method ( SEC) was established for the screening of macromolecules in traditional Chinese medicine (TCM) injections. In the experiment, TCM was collected through 0. 45μm hybrid filter and separated by SEC with MAbPac SEC-1 column. Acetonitrile was added by post-column mode with a flow rate of 0. 2 mL/min to enhance the detection sensitivity, and 25 mmol/L ammonium formate was used as the mobile phase at flow rate of 0. 2 mL/min. The charged aerosol detection was carried out with Dextran and Tween as the reference standards. The molecular weight distribution of Dextran ranged from 2500 to 133800 had a good linear relationship with the retention time. No macromolecules were detected out in the selected traditional Chinese medicine injections except Kuhuang injections.关键词
Charged aerosol detection/Liquid chromatography/Size exclusion chromatography/Macromolecule screeningKey words
Charged aerosol detection/Liquid chromatography/Size exclusion chromatography/Macromolecule screening引用本文复制引用
张婷婷,王玉,谷丹华,张俐,金燕..双三元液相色谱-串联电喷雾检测器用于中药注射剂大分子物质筛查研究[J].分析化学,2014,(12):1871-1872,2.
