分析化学Issue(7)：971-976,6.DOI:10.11895/j.issn.0253-3820.150159
纳米通道单分子检测低噪音电流放大器系统的研究
A Low Noise Amplifier System for Nanopore-based Single Molecule Analysis
摘要
Abstract
A novel amplifier system was proposed for low-noise recording of pico-ampere current in nanopore experiment (<100 pA). As an example, the amplifier system was applied in α-hemolysin based nanopore detection of DNA-PEG-DNA conjugate to record the signals of translocation and bumping events in buffer solution (1 mol/L KCl, 10 mmol/L Tris--HCl, 1 mmol/L EDTA and pH=8. 0). The amplified current signal was filtered by a 3 kHz Bessel filter and sampled by a 100 kHz analog-digital convertor. As a result, the presented amplifier system could lower the noise in recording the current. The current blockages (<10 pA) of single molecules with low amplitude were recovered due to the high signal-to-noise ratio.关键词
纳米通道/单分子检测/电流放大器/降噪Key words
Nanopore/Single molecule detection/Current amplifier/Denoising引用本文复制引用
颜秉勇,顾震,高瑞,曹婵,应佚伦,马巍,龙亿涛..纳米通道单分子检测低噪音电流放大器系统的研究[J].分析化学,2015,(7):971-976,6.基金项目
本文系国家自然科学基金委(Nos.21125522,21327807,51407078)和中央高校基本科研业务费专项资金(Nos.222201313004, WH1514049)资助项目 （Nos.21125522,21327807,51407078）
