分析化学Issue(7)：983-988,6.DOI:10.11895/j.issn.0253-3820.150039
脑死亡过程中谷氨酸与场电位的同步检测微电极阵列研究
Study of Microelectrode Array Probe for Simultaneous Detection of Glutamate and Local Field Potential during Brain Death
摘要
Abstract
High extracellular potassium can induce spreading depression-like depolarizations, elevations of extracellular glutamate and even neuronal death in normal brain. To investigate the contribution of high potassium in vivo, a microelectrode arrays ( MEAs ) probe integrated with recording sites for glutamate concentration (50í150 μm) and local field potential ( LFP) ( diameter=15 μm) was fabricated by Micro-electro-mechanical-systems ( MEMS) technologies. We implanted the MEA probe acutely in the rat brain and exposed the brain to a high potassium solution. During these multi-modal recordings, it was observed that high potassium elevated extracellular glutamate while suppressing the LFP irreversibly. This is one of the first studies in which a dual mode MEA probes is applied in vivo for neuronal death, and it is concluded that our MEA probes are capable of examining specific spatiotemporal relationships between electrical and chemical signaling in the brain.关键词
微机电系统/植入式微电极阵列/脑死亡/高钾/谷氨酸/局部场电位Key words
Implantable microelectrode array/Micro-electro-mechanical-systems/Brain death/High potassium/Glutamate/Local field potential引用本文复制引用
蔚文婧,宋轶琳,范心怡,张松,王力,徐声伟,蔡新霞..脑死亡过程中谷氨酸与场电位的同步检测微电极阵列研究[J].分析化学,2015,(7):983-988,6.基金项目
本文系国家重大科学研究计划(Nos.2011CB933202,2014CB744605)、北京市科技计划(Nos. Z141100000214002, Z141102003414014)、国家自然科学基金项目(Nos.61125105,61101048,61471342)、中科院重点项目(No. XDA06020101)资助课题 （Nos.2011CB933202,2014CB744605）
