法医学杂志Issue(3)：188-190,3.DOI:10.3969/j.issn.1004-5619.2014.03.008
氨基比林血痕预试验处理血痕后样本的DNA定量
DNA Quantification of Blood Samples Pre-treated with Pyramidon
摘要
Abstract
Objective To study DNA quantification and STR typing of samples pre-treated with pyrami-don. Methods The blood samples of ten unrelated individuals were anticoagulated in EDTA. The blood stains were made on the filter paper. The experimental groups were divided into six groups in accor-dance with the storage time, 30 min, 1 h, 3 h, 6 h, 12 h and 24 h after pre-treated with pyramidon. DNA was extracted by three methods: magnetic bead-based extraction, QIAcube DNA purification method and Chelex-100 method. The quantification of DNA was made by fluorescent quantitative PCR. STR typing was detected by PCR-STR fluorescent technology. Results In the same DNA extraction method, the sample DNA decreased gradually with times after pre-treatment with pyramidon. In the same storage time, the DNA quantification in different extraction methods had significant differences. Sixteen loci DNA typing were detected in 90.56% of samples. Conclusion Pyramidon pre-treatment could cause DNA degradation, but effective STR typing can be achieved within 24 h. The magnetic bead-based extrac-tion is the best method for STR profiling and DNA extraction.关键词
法医遗传学/氨基比林预试验/磁珠法/QIAcube DNA纯化法/Chelex-100法Key words
forensic genetics/pyramidon preliminary test/magnetic bead-based extraction/QIAcube DNA purification method/Chelex-100 method分类
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朱传红,郑道利,倪尧志,王海生,宁平,方慧,刘艳..氨基比林血痕预试验处理血痕后样本的DNA定量[J].法医学杂志,2014,(3):188-190,3.基金项目
公安部2009年应用创新项目课题 （）
