法医学杂志Issue(6)：437-440,4.DOI:10.3969/j.issn.1004-5619.2014.06.009
16个X-STR基因座的连锁不平衡检验和突变率调查
Linkage Disequilibrium and Mutation Rate Analysis of Sixteen X-STR Loci
摘要
Abstract
Objective To assess the patterns oflinkage disequilibrium (LD ) of16 STR loci on X chrom o-som e and investigate the genetic stability. Methods G enom ic DNA samples extracted from blood stains from 500 unrelated individuals and 885 lineage m em bers from E astern C hinese H an population were genotyped through m ultiplex am plification using ID typerX-16 kit by our independent research followed by capillary electrophoresis. LD was assessed by PowerM arker v3.25 software and m utation rate of every locus was analyzed. Results LD were not found at the 16 X-STR loci. A llele m utations were observed at 10 loci. A m ong them , m utation rates of DXS6809 and DXS7132 were both up to 0.004 8. Conclusion W hen the 16 X-ST R loci included in ID typerX-16 kit were used for parentage testing, product princi-ples can be applied to calculate the likelihood, but m utation should be taken into consideration in the case that the genotypes do not m eet the genetic law(especially at DXS6809 and DXS7132).关键词
法医遗传学/X染色体/短串联重复序列/连锁不平衡/突变率Key words
forensic genetics/X chrom osom e/short tandem repeats/linkage disequilibrium/m utation rate分类
社会科学引用本文复制引用
李莉,刘俊宏,朱如心,林源..16个X-STR基因座的连锁不平衡检验和突变率调查[J].法医学杂志,2014,(6):437-440,4.基金项目
上海市自然科学基金资助项目（08ZR1419800）；上海市科委科研项目 （）
