法医学杂志Issue(3)：188-190,3.DOI:10.3969/j.issn.1004-5619.2015.03.004
BAT在非离子型造影剂过敏反应中的诊断价值
Diagnostic Significance of BAT in Anaphylaxis to Non-ionic Contrast Media
摘要
Abstract
Objective To investigate the diagnostic significance of basophil activation test (B A T) in ana-phylaxis to non-ionic contrast media through testing the content of CD 63, m ast cell-carboxypeptidase A 3 (M C-CPA 3), and term inal com plem ent com plex SC5b-9 of the individuals by testing their levels in the norm al im m une group and the anaphylaxis groups to β-lactam drugs and non-ionic contrast media. Methods The CD 63 expression of basophilic granulocyte in blood w as detected by flow cytom etry. The levels of M C-CPA 3 in blood serum and SC5b-9 in blood plasm a w ere detected by ELISA . Results The CD 63 expression of basophilic granulocyte in blood, the levels of M C-CPA 3 and SC5b-9 of anaphylaxis to non-ionic contrast media and β-lactam drugs w ere significantly higher than that in norm al im m une group (P<0.05). Conclusion There is activation of basophilic granulocytes, m ast cells and com plem ent system in anaphylaxis to non-ionic contrast media. B A Tcan be used to diagnose the anaphylaxis to non-ionic contrast media.关键词
法医病理学/过敏反应/非离子型造影剂/嗜碱性粒细胞活化试验/CD63/肥大细胞羧肽酶A3/SC5b-9Key words
forensic pathology/anaphylaxis/non-ionic contrast m edia/basophil activation test/C D 63/m ast cell carboxypeptidase A 3/SC 5b-9分类
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张皓月,许素军,唐笑先,牛记军,郭相杰,高彩荣..BAT在非离子型造影剂过敏反应中的诊断价值[J].法医学杂志,2015,(3):188-190,3.基金项目
国家自然科学基金资助项目 （）
