发育医学电子杂志Issue(3)：139-145,7.
先天性纯红细胞再生障碍性贫血斑马鱼模型相关microRNA的组学研究
microRNome analysis of the Zebrafish model of diamond-blackfan anemia from Rps19 deficiency
摘要
Abstract
Objective To characterize the genome-wide microRNA profile and to explore the biological functions and probable mechanisms for microRNAs in diamond-blackfan anemia (DBA). Method Zebrafish embryos at the one-cell stage were injected with the Rps19 MO and Rps19+p53 MO. The miRNomes of these zebrafish models and control, generated by miRNA-seq, were compared for determining the microRNA profile characteristics of DBA and regulatory function of p53. Result Rps19 morphants showed an obviously ventrally bent tail and a reduction in the circulating blood cells compared with the control embryos, which were partially rescued in Rps19+p53 MO at 48 hpf. By comparing the miRNomes with control, we found that about 25% of total expressed microRNAs in Rps19 MO had significantly lower expression, which might make an influence on the development process of Zebrafish embryos. The abnormal miRNomes were partially reversed by simultaneous knockdown of p53. We determined 47 microRNAs that were potentially regulated by p53 in DBA pathologic process.Conclusion microRNAs in Zebrafish model of DBA were in an inactive state, which was partially affected by p53.关键词
纯红细胞再生障碍性贫血/microRNA/Rps19/p53/RNA-seq/斑马鱼Key words
Diamond-blackfan anemia/microRNA/Rps19/p53/RNA-seq/Zebrafish引用本文复制引用
张倩,贾海波,方向东,宋斌峰,万扬,张昭军,鲍丙浩,谢尚论,刘汉芝,竺晓凡,袁卫平..先天性纯红细胞再生障碍性贫血斑马鱼模型相关microRNA的组学研究[J].发育医学电子杂志,2014,(3):139-145,7.基金项目
国家“十二五”科技支撑计划分课题(2013BAI01B09)；国家重大科学仪器设备开发专项子课题（2011YQ03013404）；国家自然科学基金 （2013BAI01B09）
