高等学校化学学报Issue(7)：1590-1595,6.DOI:10.7503/cjcu20140045
CMC-g-DNA 接枝共聚物的合成与表征
Preparation and Characterization of the CMC-g-DNA Hybrid Copolymers
摘要
Abstract
Sodium carboxymethyl cellulose grafted DNA(CMC-g-DNA) hybrid copolymer, which can be a potential gene drug, was synthesized and characterized by both chemical and biological methods. First, the carboxyl groups on the side chain of CMC were activated by both 1-(3-dimethylaminopropyl)-3-ethylcarbo-diimide(EDC) and N-hydroxy-succinimide(NHS) in phosphate buffer at both neutral and acid condition, re-spectively. After that, the CMC-NHS reacted with amino-functionalized oligodeoxynucleotides(NH2-ODNs) to get CMC-g-ODNs grafted copolymer. Then CMC-g-ODNs were used as forward primers for the next-step poly-merase chain reaction(PCR) to generate the final CMC-g-DNA hybrid copolymer. The infrared spectrum de-tection proved that the side carboxyl groups of the CMC were substituted very well by NHS and the efficiency was higher at acid condition than neutral. The obtained CMC-g-DNA copolymers were characterized by agarose gel electrophoresis and polyacrylamide gel electrophoresis, respectively. And the results showed that DNA was grafted on CMC successfully. An interesting finding is that CMC-g-DNA moved faster than control DNA in aga-rose gel electrophoresis, while it migrated slower than control polyacrylamide gel electrophoresis.关键词
脱氧核糖核酸(DNA)/羧甲基纤维素钠/接枝共聚物/凝胶电泳/聚合酶链式反应/迁移速率/基因载体Key words
DNA/Sodium carboxymethyl cellulose ( CMC )/Hybrid copolymer/Gel electrophoresis/Polymerase chain reaction(PCR)/Migrated speed/Gene combination分类
化学化工引用本文复制引用
李敏,邢朝晖,田艳芝,姜勇..CMC-g-DNA 接枝共聚物的合成与表征[J].高等学校化学学报,2014,(7):1590-1595,6.基金项目
国家自然科学基金(批准号:21174029)和教育部留学回国人员科研启动基金资助. Supported by the National Natural Science Foundation of China(No.21174029) and the Project Sponsored by the Scientific Research Foun-dation for the Returned Overseas Chinese Scholars, State Education Ministry, China （批准号:21174029）
