高等学校化学学报Issue(8)：1658-1664,7.DOI:10.7503/cjcu20140336
玉型胶原膜的类液晶结构对人脐带间充质干细胞黏附、增殖和分化性能的影响
Effects of Collagen Films with Liquid Crystal-liked Ordered Structure on Adhesion, Proliferation and Differentiation of Human Umbilical Cord Mesenchymal Stem Cells
摘要
Abstract
To mimic the ordered arrangement of typeⅠcollagen in natural tissue, a collagen films with liquid crystal-liked ordered surface were fabricated utilizing the liquid crystal state of concentrated collagen. And the effects of the topography on the behavior of human umbilical cord mesenchymal stem cells( hUCMSCs) were studied in vitro. The results showed that the collagen fibrils arranged directly on the surface of the collagen films when the concentration was over 120 mg/mL. This ordered structure improved significantly the adhesion and proliferation of hUCMSCs. In addition, it also increased the expression of osteogenic gene such as ALP, Collagen Ⅰ , RUNX2, Osterix, OCN and OPN, which meant that hUCMSCs had a stronger differentiation po-tency on the ordered surface than on the unordered surface.关键词
Ⅰ型胶原/类液晶有序结构/人脐带间充质干细胞/成骨分化Key words
Type Ⅰ collagen/Liquid crystal-liked ordered structure/Human umbilical cord mesenchymal stem cell( hUCMSC)/Osteogenic differentiation分类
化学化工引用本文复制引用
曾庆慧,李娜,田冶,吴狄,赵要武,李立华,周长忍..玉型胶原膜的类液晶结构对人脐带间充质干细胞黏附、增殖和分化性能的影响[J].高等学校化学学报,2014,(8):1658-1664,7.基金项目
国家自然科学基金(批准号:31270021,81171459)、广东省自然科学基金(批准号：S2013010013955)、珠江新星专项项目(批准号:2011J2200037)和暨南大学科研创新基金(批准号:21612320)资助.Supported by the National Natural Science Foundation of China(Nos.31270021,81171459), the Natural Science Foundation of Guangdong Province, China ( No. S2013010013955), the Project of Science & Technology Star of Zhujiang City, Guangdong Province, China ( No.2011J2200037) and the Scientific Research Foundation of Jinan University Innovation of China(No.21612320) （批准号:31270021,81171459）
