高等学校化学学报Issue(9)：1883-1888,6.DOI:10.7503/cjcu20140308
基于核磁共振氢谱代谢组学研究黄连解毒汤对胰岛素抵抗大鼠棕色脂肪组织代谢组的影响
Effects of Huanglian Jiedu Decoction on the Metabolic Features of Brown Adipose Tissue Extracts in Fructose-induced Insulin Resistance Model Based on NMR Metabonomics
摘要
Abstract
With the application of 1H nuclear magnetic resonance(1 H NMR) spectroscopy, we studied the effect of Huanglian Jiedu decoction( HJD) on the metabonomics of brown adipose tissue extracts in high fruc-tose-induced insulin resistance model. 32 Wistar rats were divided into four groups, i. e. , controls, model group, positive drug group and HJD treated group, 8 in each group. The last three groups were given 100 g/L fructose water for 28 d to establish insulin resistance model, normal control group was given the equal volume of pure water at the same time. After 28 d, four groups of rats were given 100 g/L fructose water continuously. Rats in positive drug group were administered orally atorvastain at a dose of 10 mg/( kg·d) , and rats in HJD treated group were given by gavage with HJD water. The control and model groups were given by gavage with a certain volume of saline solution, and the experiment lasted 56 d. Brown adipose tissues were obtained and 1 H NMR spectra of each sample was performed and analyzed by principal component analysis( PCA) method. Compared with the control group, lactate, alanine, choline, phosphocholine/glycerol phosphocholine ( PC/GPC) , creatine/creatinine, taurine and inosine increased in the model and lipid decreased. In comparison with model group, the myo-inositol was increased in HJD group, and HJD had reversed the metabolites in the model group. HJD can regulate the body’ s energy metabolism and reduce the damaged cell membrances and the injury of liver and kidney. Therefore, this study elucidates the metabolic mechanism of HJD on insulin re-sistance( IR) .关键词
核磁共振氢谱/代谢组学/黄连解毒汤/胰岛素抵抗Key words
1 H NMR/Metabonomics/Huanglian Jiedu decoction/Insulin resistance分类
化学化工引用本文复制引用
杨永霞,王琳琳,郑凌云,王淑美,黄榕波,张磊,黄耀庭..基于核磁共振氢谱代谢组学研究黄连解毒汤对胰岛素抵抗大鼠棕色脂肪组织代谢组的影响[J].高等学校化学学报,2014,(9):1883-1888,6.基金项目
国家自然科学基金(批准号:21005022)资助. Supported by the National Natural Science Foundation of China(No.21005022) （批准号:21005022）
