高等学校化学学报Issue(11)：2325-2330,6.DOI:10.7503/cjcu20140575
10-羟基喜树碱的光谱性质、抗肿瘤活性及应用于细胞标记研究
Spectral Properties and Antitumor Activity of 10-Hydroxycamptothecin and Its Application in Cell Labeling
摘要
Abstract
The spectral properties of 10-hydroxycamptothecin( HCPT) were investigated by UV-Vis spectro-photometer, fluorescence spectrophotometer and fourier transform infrared spectroscopy. The antiproliferative activities of HCPT against three tumor cell lines, human cervical can-cer cell line ( HeLa ) , human breast cancer cell line(MCF-7) and human fibrosarcoma cell line(HT1080), were determined using an MTT assay, and the IC50 values of HCPT to the three tumor cell lines were 16. 37, 16. 73 and 19. 24μg/mL, respectively. The growth inhibitory effect of HCPT on a normal cell line, HEK293T(human embryonic kidney cells 293T), was also investigated, the highest inhibitory rate reached 88. 72% at an HCPT concentration of 40μg/mL, in-dicating that the normal cell line HEK293 T is more sensitive to HCPT in comparison to the three tumor cell lines. Using HeLa cell line as a model, Annexin V-FITC apoptosis detection kit was employed to demonstrate the antitumor mechanism of HCPT. Almost all the cells were simultaneously stained by Annexin V-FITC and PI, the cell membrane was green while the nucleus was red, which indicated that HCPT induced the late apoptosis process of HeLa cells. The distribution of HCPT in HeLa cells was observed by fluorescence micros-copy, which provides scientific basis for its application in cell labeling.关键词
10-羟基喜树碱/光谱性质/抗肿瘤活性/细胞凋亡/细胞标记Key words
10-Hydroxycamptothecin/Spectral property/Antitumor activity/Apoptosis/Cell labeling分类
化学化工引用本文复制引用
丁国斌,李彬春,郭轶,徐力..10-羟基喜树碱的光谱性质、抗肿瘤活性及应用于细胞标记研究[J].高等学校化学学报,2014,(11):2325-2330,6.基金项目
国家自然科学基金(批准号:81271697,30970719)、山西省基础研究项目青年科技研究基金(批准号:2013021014-1)和山西大学科研启动资金(批准号:020651901007)资助 （批准号:81271697,30970719）
