高等学校化学学报Issue(4)：631-637,7.DOI:10.7503/cjcu20140978
纳米壳聚糖修饰的开管毛细管电色谱柱的制备及对碱性蛋白质的分离
Preparation of Open Tubular Capillary Electrochromatography Column with Nano-chitosan Coating and Its Application for Basic Proteins Analyzing
摘要
Abstract
Nano-chitosan particles ( N-CS ) prepared by ionic crosslinking technology were coated onto the inner wall of fused silica capillary via electrostatic adsorption to get a nano-chitosan coating capillary column. N-CS particles were successfully coated on the inner wall of the capillary as hillocks observed by field emission scanning electron microscopy. The electroosmotic flow( EOF) was tested in buffer at different pH values and compared to an uncoated capillary. The result showed that it was definitely inhibited after modification and re-versed when pH<4. 7 . It was also found that EOF RSD of the prepared column was less than 6% in different acidity buffer and RSD of intra-day, inter-day and column to column were 1. 46%, 4. 64% and 14. 4%, re-spectively. Those results indicated that the stability of N-CS coated column was good. The chromatographic behavior of the coated column was tested by separating three neutral analytes namely toluene, phenol and thio-urea, the eluotropic sequence was consistent with their polarity, which indicated the nano-chitosan coatings played the role of hydrophilic stationary phase. Three basic proteins including lysosomes, cytochrome C and ribonuclease A were successfully separated with the resulting column, and their column efficiency were 39481, 42610 and 245373 Plate/m, respectively. The results showed that the adsorption between basic proteins and fused silica capillary is prevented effectively in the N-CS coated capillary.关键词
纳米壳聚糖/开管毛细管电色谱/碱性蛋白质分离Key words
Nano-chitosan particle/Open tubular capillary electrochromatography/Separation of basic proteins分类
化学化工引用本文复制引用
张欢欢,陈继涢,周孙英..纳米壳聚糖修饰的开管毛细管电色谱柱的制备及对碱性蛋白质的分离[J].高等学校化学学报,2015,(4):631-637,7.基金项目
福建省自然科学基金(批准号:2013J01037)资助.覮 Supported by the Natural Science Foundation of Fujian Province, China(No.2013J01037) （批准号:2013J01037）
