衍生于双氰基二苯代乙烯的用于活体成像的双光子荧光锌离子探针
Dicyanostilbene-derived Two-photon Fluorescence Probe for Free Zinc Ions in Live Cells and Living Tissues
摘要
Abstract
A novel two-photon fluorescence probe for Zn2+ derived from dicyanostilbene as a two-photon fluorophore and 4-( pyridine-2-ylmethyl) piperazine as a novel Zn2+ligand was developed, and its structure was characterized by 1 H NMR, IR and elemental analysis. By UV-Vis absorption and absorption-titration experi-ments, one-and two-photon excited fluorescence experiments in femtosecond pulses, as well as cell-imaging and tissue-imaging experiments, the probe showed a 72. 5-fold fluorescence enhancement in response to Zn2+, a large two-photon action cross-section(580 GM), a noncytotoxic effect, and pH insensitivity in the biolo-gically relevant range, with a dissociation constant(KTPd ) of (0. 52±0. 01) μmol/L. The probe could detect free Zn2+ ions selectively in live cells for about 1500 s and in living tissues at a depth of 80—150 μm without interference from other metal ions or membrane-bound probes.关键词
双光子荧光探针/锌离子/活体成像/双光子发射截面Key words
Two-photon fluorescence probe/Zinc ion/Live-imaging/Two-photon absorption across-section分类
化学化工引用本文复制引用
黄池宝,梁兴,曾启华,陈华仕,曾伯平,易道生,陈晓远..衍生于双氰基二苯代乙烯的用于活体成像的双光子荧光锌离子探针[J].高等学校化学学报,2015,(4):646-653,8.基金项目
中国博士后科学基金(批准号:20100471224)、广东省自然科学基金(批准号：s2011040000536)、湖南省科技计划项目(批准号:2011RS4020)、深圳大学省部重点实验室开放基金项目(批准号:201202)、韶关市科技计划项目(批准号:2011CX/K19)和贵州省教育厅自然科学重点研究项目(批准号:黔教合KY字[2014]296).覮 Supported by the China Postdoctoral Science Foundation Funded Project ( No.20100471224), the Guangdong Provincial Natural Science Foundation, China(No. S2011040000536), the Planned Science and Technology Project of Hunan Province, China(No.2011RS4020), the Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, China(No.201202), the Planned Science and Technology Project of Shaoguan, China(No.2011CX/K19) and the Natural Science Key Research Project of Department of Education of Guizhou Province, China(No. Guizhou Province Office of Education Contract KY [2014]296) （批准号:20100471224）
