高等学校化学学报Issue(6)：1061-1066,6.DOI:10.7503/cjcu20150016
基于聚多巴胺纳米粒子的荧光共振能量转移法检测microRNA
Polydopamine Nanoparticle-based Fluorescence Resonance Energy Transfer Assay for microRNA Detection
摘要
Abstract
A simple, rapid and low-cost polydopamine nanoparticle( PDA NP)-based fluorescence resonance energy transfer( FRET) assay with deoxyribonucleaseⅠ( DNaseⅠ)-assisted target recycling signal amplifica-tion was developed for microRNA ( miRNA ) detection. Under the optimized experimental conditions, the recovery ratio of Cy5 fluorescence intensity increased linearly with logarithm of miR-21 concentration from 10 pmol/L to 100 nmol/L, with a detection limit of 7 pmol/L. Moreover, satisfactory results were obtained while the proposed method was applied to detect miRNA in 10% human serum.关键词
聚多巴胺纳米粒子/微小核糖核酸/脱氧核糖核酸酶Ⅰ/荧光共振能量转移Key words
Polydopamine nanoparticle/microRNA/DNaseⅠ/Fluorescence resonance energy transfer分类
化学化工引用本文复制引用
马立娜,柳扶,高嘉雪,王振新..基于聚多巴胺纳米粒子的荧光共振能量转移法检测microRNA[J].高等学校化学学报,2015,(6):1061-1066,6.基金项目
国家自然科学基金(批准号:21127010)资助 （批准号:21127010）
