广东医学Issue(8)：1143-1145,3.
糜酶对犬血管平滑肌细胞增殖的影响
Effects of chymase on proliferation of cultured dog vascular smooth muscle cells
摘要
Abstract
Objective To investigate the effect of chymase on the cell proliferation and angiotensin Ⅱ( AngⅡ) generation of dog vascular smooth muscle cells .Methods The dog artery smooth muscle cells were cultured and divided into four groups:Control group, Angiotensin Ⅰ(Ang Ⅰ) group, Captopril group and Chymostatin group .Cell counting was performed by trypan blues staining , while the ratio of light density by MTT and Ang Ⅱconcentration were measured in the culture medium .Results The cell counts were significantly reduced in Captopril group and Chymostatin group com -pared with Ang Ⅰgroup (P<0.05).The cell proliferation of Chymostatin group was significantly inhibited (P<0.05). Moreover, the concentration of Ang Ⅱin Chymostatin group was reduced significantly (P<0.05).Conclusion Chymase may play an important role in the generation of Ang Ⅱand the proliferation of cultured dog vascular smooth muscle cells , via which chymostatin may inhibit cell proliferation .关键词
糜酶/犬血管平滑肌细胞/血管紧张素Ⅱ/细胞增殖Key words
chymase/dog smooth muscle cell/angiotensin Ⅱ/cell proliferation引用本文复制引用
孙成林,马骏,刘宗凤,白英奎,赵延鹏,张绍军..糜酶对犬血管平滑肌细胞增殖的影响[J].广东医学,2014,(8):1143-1145,3.基金项目
辽宁省科学技术计划项目 （）
