广东医学Issue(11)：1654-1658,5.
E2F -1过表达对人胃癌 SGC -7901细胞增殖的影响及其机制
Effects of E2F - 1 overexpression on the proliferation of human gastric cancer cell line SGC - 7901 and its mecha-nism
摘要
Abstract
Objective To investigate the effect of E2F -1 overexpression on the proliferation,growth and cell cy-cle of human gastric cancer cell line SGC -7901 and the molecular mechanism. Methods Humans gastric cancer SGC -7901 cells were infected by E2F - 1 recombinant lentiviral particles(LV - E2F - 1 - GFP),as experimental group(LV -E2F - 1 - GFP group),while control lentiviral particles(LV - GFP)infected human gastric cancer SGC - 7901 cells,as a negative control group(LV - GFP group)and blank control group was cultured conventionally without any treatment. CCK - 8 were used to detect cell proliferation activity,while flow cytometry was used for assessment of cell cycle distribu-tion. Semi - quantitative reserve transcription - polymerase chain reaction(RT - PCR)and Western blot were applied for measurement of mRNA and protein expression of Skp2,Bax,Bcl - 2,CylinD1 and Survivin. Results Compared with LV - GFP group and blank control group,significant lower proliferation activity(P < 0. 05),increased G0 / G1 phase pro-portion(P < 0. 05),down - regulation of mRNA and protein of Skp2,Bcl - 2,CylinD1 and Survivin(P < 0. 05),up -regulation of Bax mRNA and protein(P < 0. 05),were revealed LV - E2F - 1 - GFP group. Furthermore,there was no significant difference between LV - GFP group and blank control group(P > 0. 05). Conclusion Lentivirus - mediated overexpression of E2F - 1 induces growth inhibition of cancer cells,and cell cycle arrest at G0 / G1 phase;probably via up - regulation of Bax and down - regulation of Skp2,Survivin,Bcl - 2 and CylinD1.关键词
胃癌/SGC - 7901 细胞/E2F - 1/过表达Key words
gastric cancer/SGC - 7901 cells/E2F - 1/overexpression引用本文复制引用
曹稳珑,韦尉元,张笑石,罗文,严林海,谢玉波,肖强..E2F -1过表达对人胃癌 SGC -7901细胞增殖的影响及其机制[J].广东医学,2014,(11):1654-1658,5.基金项目
国家自然科学基金资助项目（编号30860273），广西科学研究与技术开发计划项目（编号桂科攻10124001A -22），广西自然科学基金资助项目 （）
