广东医学Issue(17)：2656-2658,3.
microRNA-451和辅助性T淋巴细胞17亚群在病毒性心肌炎小鼠中的变化及意义
Alteration and significance of microRNA-451 and Th17 cells in mice with coxsackie virus induced myocarditis
摘要
Abstract
Objective To observe microRNA-451 (miR-451) and T helper cells 17 (Th17) subsets in mice with viral myocarditis induced by coxsackie virus B3 ( CVB3 ) , thus to investigate the roles of miR-451 and Th17 in patho-genesis of viral myocarditis ( VMC) in mice.Methods VMC mouse models were constructed with peritoneally injection of CVB3 in Balb/c male mice, while phosphate buffer was used as control.Flow cytometry analysis was used to evaluate the frequencies of Th17 subsets in CD4 +T cells on Day 0, 7, 14 and 28 after virus injection.The levels of miR-451 in mice myocardial tissues were determined by quantitative polymerase chain reaction.Results Statistical differences in the proportion of Th17 cells were observed between VMC mice and controls at different time points ( P<0.05 ) .In VMC mice, the proportion of spleen Th17 cells was significantly increased on Day 7 than Day 0, and reached the peaked on Day 28.The expression levels of miR-451 in VMC mice were significantly reduced since the Day 7, and reached the lowest point on Day 14.Significant reduction in miR-451 was observed in VMC mice through the study (P<0.05).No signifi-cant correlation was revealed between the expression of miR-451 and Th17 cells.Conclusion miR-451 and Th17 cells may be involved in process of CVB3 -induced VMC in mice.miR-451 may play a role in the autoimmune patho-genesis of mice with VMC but not through Th17 cells.关键词
microRNA-451/辅助性T淋巴细胞17亚群/病毒性心肌炎Key words
microRNA-451/T helper cells 17/viral myocarditis引用本文复制引用
刘艳丽,伍伟锋..microRNA-451和辅助性T淋巴细胞17亚群在病毒性心肌炎小鼠中的变化及意义[J].广东医学,2014,(17):2656-2658,3.基金项目
国家自然科学基金资助项目 （）
