干旱地区农业研究Issue(3)：15-23,9.
干旱胁迫对3种苹果属植物叶片解剖结构、微形态特征及叶绿体超微结构的影响
Influence of drought stress on leaf anatomical structure and micro-morphology traits and chloroplast ultrastructure of three Malus species
摘要
Abstract
The changes of leaf anatomical structure and several micro-morphology features (stomatal density and size ,and the upper and lower cuticle thickness ) as well as chloroplast ultrastructure were observed for three Malus plants ,drought-tolerant M . prunifolia and M . sieversii , and drought-sensitive M . hupehensis , grown under mild , moderate and severe water deficit ,using the semi-thin/ultrathin section ,light microscope ,scanning electron microscopy (SEM ) ,and transmission electron microscopy (TEM ) methods . For the three genotypes , compared with well watered plants ,the leaf mesophyll contents and palisade tissue thickness as well as cell tightness rate (CTR) in drought-stressed plants significantly decreased ( P<0 .05 ) ,while the sponge tissue thickness and scattered rate (SR ) significantly in-creased ( P<0 .05 ) .SEM photos indicated that the stomatal density in young leaves of drought-stressed plants increased ( P<0 .05 ) ,while the stomata width ,stomatal opening rate ,and the relative opening degree of stomata decreased .TEM analysis showed that the upper and lower cuticle thickness of M . prunifolia and M . sieversii increased ,whereas those of M . hupehensis increased first and then decreased with prolonged drought treatment ,swelling chloroplast ,decreased the number of larger starch granules ,and loosing grana and thylakoid were the typical leaf ultrastructure for medium water stress .Under severe water deficit ,the chloroplasts were round in a shape ,with more damaged structure of membranes , and an extensive vacuolization and disorganization of thylakoid . However , the degree of drought-induced damage was smaller in M . prunifolia and M . sieversii plants as compared to M . hupehensis plants ,duo to the ability to maintain cell integrity .关键词
苹果属/叶片解剖结构/角质层/气孔/叶绿体超微结构Key words
Malus/leaf anatomical structure/cuticle/stomata/chloroplast ultrastructure分类
农业科技引用本文复制引用
王顺才,邹养军,马锋旺..干旱胁迫对3种苹果属植物叶片解剖结构、微形态特征及叶绿体超微结构的影响[J].干旱地区农业研究,2014,(3):15-23,9.基金项目
国家苹果产业技术体系（CARS -28）；甘肃省科技厅农业科技成果转化资金计划（1105NCNE109）；天水师范学院科研资助项目 （）
