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转基因小麦抗旱性鉴定及相关指标灰色关联度分析

罗影 王炜 赵军 王剑虹 裴怀弟 张斌科 叶春雷 李进京 欧巧明 王红梅

干旱地区农业研究Issue(1):48-53,6.
干旱地区农业研究Issue(1):48-53,6.DOI:10.16302/j.cnki.1000-7601.2015.01.008

转基因小麦抗旱性鉴定及相关指标灰色关联度分析

Characterization of drought resistance in transgenic wheat lines and grey correlation analyses of related traits

罗影 1王炜 2赵军 3王剑虹 4裴怀弟 2张斌科 5叶春雷 2李进京 2欧巧明 2王红梅2

作者信息

  • 1. 甘肃农业大学资源与环境学院,甘肃 兰州 730070
  • 2. 甘肃省农业科学院生物技术研究所,甘肃 兰州 730070
  • 3. 中国农业科学院生物技术研究所/国家农作物基因资源和基因改良重大科学工程,北京 100081
  • 4. 兰州职业技术学院生物工程系,甘肃 兰州 730070
  • 5. 甘肃农业大学农学院,甘肃 兰州 730070
  • 折叠

摘要

Abstract

A total of 11 traits ,including 7 agronomic traits and 4 biochemical traits ,was measured in nine ABP9-transgenic spring wheat lines and their donor parent Ningchun 4 that were grown in rain-fed (drought stress ) and well-wa-tered environments .Drought resistance capabilities of these materials were evaluated comprehensively by employing the methods of subordinate function and drought-resistance measure value ( D value ) . Correlation degrees between these traits and drought resistance indexes were analyzed and evaluated using grey relational grade analysis method .The results showed that the drought resistance of the 6 transgenic wheat lines was better than that of their donor parent Ningchun 4 . In particular ,3 lines even exhibited stronger drought resistance than a non-transgenic spring wheat cultivar Dingxi 35 , which was commonly used as a control for testing drought resistance of drought-land spring wheat in the northwest Chi-na .Results of grey relational grade analyses indicated that the correlation degrees between these traits and drought resis-tance indexes were ranked from high to low in the following order:plant height ,single spike weight ,spike length ,SOD activity ,number of kernel per spike ,effective tillers ,POD activity ,1000-kernel weight ,proline content ,and then MDA content .

关键词

转基因小麦/ABP9基因/抗旱性鉴定

Key words

wheat/ABP9 gene/drought resistance parameters/subordinate function coefficients/grey correlative analysis

分类

农业科技

引用本文复制引用

罗影,王炜,赵军,王剑虹,裴怀弟,张斌科,叶春雷,李进京,欧巧明,王红梅..转基因小麦抗旱性鉴定及相关指标灰色关联度分析[J].干旱地区农业研究,2015,(1):48-53,6.

基金项目

国家转基因生物新品种培育重大专项(2009ZX08005-013B );甘肃省科技支撑项目(1104NKCA073);甘肃省农业科学院农业科技创新专项 ()

干旱地区农业研究

OACSCDCSTPCD

1000-7601

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