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苦荞遗传多样性分析与核心种质筛选

徐笑宇 方正武 杨璞 高金锋 王鹏科 冯佰利

干旱地区农业研究Issue(1):268-277,10.
干旱地区农业研究Issue(1):268-277,10.DOI:10.16302/j.cnki.1000-7601.2015.01.044

苦荞遗传多样性分析与核心种质筛选

Genetic diversity analysis of tartary buckwheat and selection of core collections

徐笑宇 1方正武 1杨璞 2高金锋 1王鹏科 1冯佰利1

作者信息

  • 1. 旱区作物逆境生物学国家重点试验室/西北农林科技大学,陕西 杨凌 712100
  • 2. 长江大学农学院,湖北 荆州 434025
  • 折叠

摘要

Abstract

SSR marker was utilized in this study to analyze the genetic diversity of 210 tartary buckwheat accessions from Tibet ,Shaanxi ,Sichuan and Guizhou provinces .Results had indicated that among the overall 50 SSR primers , there were 16 primers demonstrating good polymorphisms .178 bands were obtained and 66 .3% of them (118 ) were polymorphic bands .The genetic similarity levels of 210 accessions ranged from 0 .62 to 0 .98 ,exhibiting an average value of 0 .80 .At the similarity level of 0 .88 ,these tartary buckwheat varieties could be clustered into five groups .According to the ultimate dendrogram ,these tartary buckwheat accessions which came from the same planting region had presented a similar clustering tendency ,reflecting that the genetic information of tartary buckwheat might be affected remarkably by its geographic distribution .Moreover ,41 accessions clustered in a V group which came from Tibet (27 ) ,Shaanxi (8 ) , and Guizhou (6) provinces showed abundant genetic diversity .Compared with other accessions ,these 41 germplasms possessed not only large genetic differences ,but also a wide range of genetic origins .Thereby it is reasonable to choose them as the foundation for selections of core collections in tartary buckwheat .

关键词

苦荞/遗传多样性/核心种质/SSR

Key words

tartary buckwheat/genetic diversity/core collections/SSR

分类

农业科技

引用本文复制引用

徐笑宇,方正武,杨璞,高金锋,王鹏科,冯佰利..苦荞遗传多样性分析与核心种质筛选[J].干旱地区农业研究,2015,(1):268-277,10.

基金项目

国家自然科学基金项目(31071472);陕西省科技攻关(2012K01-09)及陕西省小杂粮产业技术体系资助 ()

干旱地区农业研究

OACSCDCSTPCD

1000-7601

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