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白菜型冬春性油菜杂交后代抗寒性变异分析

杨刚 董红业 王月 王丽萍 孙万仓 曾秀存 刘自刚 李学才 方彦 武军艳 赵艳宁

干旱地区农业研究Issue(2):95-101,7.
干旱地区农业研究Issue(2):95-101,7.DOI:10.16302/j.cnki.1000-7601.2015.02.016

白菜型冬春性油菜杂交后代抗寒性变异分析

Analyses of cold tolerance variations in filial generations of winter and spring turnip rape (Brassica rapa L .)

杨刚 1董红业 1王月 1王丽萍 1孙万仓 1曾秀存 1刘自刚 1李学才 1方彦 1武军艳 1赵艳宁1

作者信息

  • 1. 甘肃省油菜工程技术研究中心,甘肃省干旱生境作物重点实验室/甘肃省作物遗传改良与种质创新重点实验室,甘肃农业大学农学院,甘肃 兰州 730070
  • 折叠

摘要

Abstract

The winter and spring turnip rape parents (Longyou 7 ,Longyou 9 and Parkland) and their filial genera-tions ,significantly differing in cold tolerance and quality ,were comprehensively analyzed .Cold tolerance variations were examined on the basis of five physiological indexes and overwintering rates before and after cooling .The results showed that the physiological indexes of all individuals were increased after cooling .The SOD ,POD ,CAT ,soluble protein and MDA values were 131 .16 ~ 489 .50 U・g - 1 ,8 .59 ~ 68 .44 U・g - 1 ,2 .63 ~ 20 .85 U・g - 1 ,2 .53 ~ 7 .80 mg・g - 1 and 3 .69 ~ 13 .67 mg・g - 1 before cooling ,but reached to 290 .87 ~ 796 .88 U・g - 1 ,22 .63 ~ 101 .5 U・g - 1 ,8 .89 ~ 48 .30 U・g - 1 ,4 .46 ~ 10 .68 mg・g - 1 and 5 .77 ~ 21 .25 mg・g - 1 respectively after the treatment .In addition ,physiological in-dexes and overwintering rates were segregated .The variation of physiological coefficient in F1 generation was small ,and the overwintering rates of filial generations of winter turnip rape for female parents (70 .27% ) were higher than those of the spring parent for the female parent (64 .48% ) .The variations in F2 and BC1 generations were large ,and overwinter-ing rates of a group of winter parents for the recurrent parent (71 .43% ~ 76 .19% ) were significantly higher than the groups of spring parents as the recurrent parent (38 .46% ~ 43 .75% ) in BC1 .It was further found that significant corre-lations between the five physiological characteristics and overwintering rates could be identified .Correlation coefficients were 0 .597 ,0 .757 ,0 .665 ,0 .567 and -0 .663 respectively .In conclusion ,it was feasible to analyze cold tolerance characteristics using these physiological indexes ,which could reveal the differences of cold tolerance indexes and over-wintering rates in different filial generations ,and could provide good base materials for quality improvement of winter turnip rape .

关键词

白菜型油菜/杂交后代/抗寒性/生理生化特性/越冬率

Key words

Brassica rapa L ./Filial generations/Cold tolerance/Physiological indexes/Overwintering rates

分类

农业科技

引用本文复制引用

杨刚,董红业,王月,王丽萍,孙万仓,曾秀存,刘自刚,李学才,方彦,武军艳,赵艳宁..白菜型冬春性油菜杂交后代抗寒性变异分析[J].干旱地区农业研究,2015,(2):95-101,7.

基金项目

国家“863”高技术研究发展计划(2011AA10A104);国家“973”重点基础研究发展计划(2015CB150206);国家公益性行业(农业)科研专项(200903002-04;现代农业产业技术体系建设专项(CARS -13);国家自然基金(31460356);国家农业科技成果转化项目 ()

干旱地区农业研究

OACSCDCSTPCD

1000-7601

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