干旱地区农业研究Issue(3)：90-95,6.DOI:10.7606/j.issn.1000-7601.2015.03.15
马铃薯黑痣病生防菌的筛选和鉴定及其生长条件的研究
Isolation and identification of antagonistic strains against black scurf of potato and conditions for their growth
摘要
Abstract
Bacteria with antagonistic activity against potato black scurf pathogen ( Rhizoctonia solani ) were isolated from the rhizosphere soils with different years of continuous cropping in hill farm of Jingtai county ,Gansu Province .An-tagonistic bacteria were isolated from soil through dilution plates and antagonistic plates . Based on the morphological characteristics ,physiological and biochemical tests ,and 16SrDNA sequencing analysis were performed .Single factor ex-periment design was carried out to discuss the growth conditions of strain G 1 .One of the isolated strains ,G1 ,which showed the strongest antagonism ,was selected from 108 bacterial strains . It produced an inhibition zone with 6 .0mm width and > 2 .5cm diameter ,and an inhibitory rate at 66 .7% through confronting incubation .According to the mor-phological characteristics ,physiological and biochemical tests ,and 16SrDNA sequence analyses ,it was preliminarily i-dentified to be Paenibacillus sp .In addition ,a single factor experiment design was carried out to examine the optimal growth conditions for G1 .The results showed that the bacteria could grow at 4℃ ~45℃ with pH 5~10 and salt concen-tration 0 to 50 g·L-1 .The optimal conditions for G1 growth was found to be 37℃ inoculation with pH at 7 using sucrose and peptone as carbon and nitrogen sources respectively ,as well as with a salt concentration at 10 g·L-1 .In conclusion , the newly selected Paenibacillus sp .G1 showed broad inhibition zone ,with a stable effect against potato black scurf pathogen ( Rhizoctonia solani ) .The growth condition for G1 was similar to those for potato growth ,owning necessary cri-teria as a good biocontrol strain .关键词
马铃薯/黑痣病/拮抗菌/筛选鉴定/生长条件Key words
potato/black scurf/antagonistic bacteria/isolation and identification/growth condition分类
农业科技引用本文复制引用
关小敏,孟品品,刘星,邱慧珍,张文明,海龙,张俊莲,王蒂,沈其荣..马铃薯黑痣病生防菌的筛选和鉴定及其生长条件的研究[J].干旱地区农业研究,2015,(3):90-95,6.基金项目
公益性行业（农业）科研专项（201103004）；国家自然科学基金（31360500）；国家科技支撑计划（2012BAD06B03）；甘肃省科技重大专项 （）
