干旱地区农业研究Issue(3)：153-158,6.DOI:10.7606/j.issn.1000-7601.2015.03.25
甘草愈伤组织蛋白质组双向电泳技术的建立
Establishment and optimization of two-dimensional gel electrophoresis for proteome of Glycyrrhiza uralensis
摘要
Abstract
Callus of Glycyrrhiza uralensis was used in this research for extraction of total proteins ,SDS -PAGE , loading quantity and staining method ,allowing optimization of the two-dimensional gel electrophoresis (2D-E) technolo-gy .The results showed that improved acetone precipitation could increase the protein contents and image resolution of gel electrophoresis .There were 1 132 protein spots identified using silver staining when the loading quantity of the sample protein was 450 μg/(17 cm IPG trip ) .This research developed a 2D-E method applicable for protein analysis of Gly-cyrrhiz a uralensis callus .关键词
甘草/愈伤组织/双向电泳/蛋白质组学Key words
Glycyrrhiza uralensis callus/two-dimensional gel electrophoresis/proteomics分类
农业科技引用本文复制引用
曹君迈,张欣..甘草愈伤组织蛋白质组双向电泳技术的建立[J].干旱地区农业研究,2015,(3):153-158,6.基金项目
宁夏自然科学基金资助项目“甘草脱分化过程中细胞结构变化的研究” （）
