干旱地区农业研究Issue(4)：286-290,5.DOI:10.7606/j.issn.1000-7601.2015.04.43
拟南芥诱导型启动子 rd29A 的克隆及其功能鉴定
Cloning and functional characterization of an inducible promoter rd29A from Arabidopsis thaliana
摘要
Abstract
To improve drought resistance of crops ,inducible promoter rd29A was cloned from Arabidopsis thaliana by PCR method .Sequencing analysis indicated that the cloned fragment showed 99 .47% identity to the published se-quence (D13044 ) .An rd29a driving GUS expression vector pBI121-rd29-GUS was constructed by DNA recombinant technology .By Agrobacterium-mediated transformation ,pBI121-rd29-GUS was transformed into tobacco .The func-tion of rd29A promoter was identified through the expression of GUS protein in transgenic tobacco .GUS activity in trans-genic tobacco leaf showed that the rd29A promoter could drive efficient expression of the target gene . rd29A promoter could be used in subsequent transgenic study of drought resistance in potato .关键词
rd29A启动子/抗逆性/GUS基因Key words
rd29A promoter/stress resistance/GUS gene分类
农业科技引用本文复制引用
齐恩芳,贾小霞,马胜,国宏,胡新元,龚成文,王一航,李建武..拟南芥诱导型启动子 rd29A 的克隆及其功能鉴定[J].干旱地区农业研究,2015,(4):286-290,5.基金项目
甘肃省农业科学院中青年基金项目（2014GAAS20）；国家自然科学基金 （）
