赣南医学院学报Issue(4)：498-501,4.
中度低温对肠上皮细胞屏障氧化应激损伤后分泌炎症因子的影响及其临床意义
Effect of Moderate Hypothermia on Inflammatory Cytokines Secretion After Intestinal Epithelial Cell Barrier Injured by Oxidative Stress and Its Clinical Significance
摘要
Abstract
Objective:To illuminate the effect of moderate hypothermia on inflammatory cytokine expression and its clinical significance by detecting inflammatory cytokine(TNF-α、IL-1β、IL-6)expression levels after the intestinal epithelial cell barrier injured by oxidative stress. Methods:The Intestinal epithelial cell barrier model in vitro was established by Caco-2 cells,divided into moderate hypothermia group,normal temperature group and sub-hypothermia group. The expression levels of mRNA in the cells were examined by reverse transcription PCR and rea1 time PCR,and inflammatory cytokine in the cultural supernatant was measured by ELISA. Results:After the intestinal epithelial cell barrier injured by oxidative stress,the mRNA in inflammatory cytokines in moderate hypothermia group and the mass concentration of inflammatory cytokines in the cultural supernatant were significantly lower than those in normal temperature group and sub-hypothermia group. Conclusion:Moderate hypothermia can inhibit the secretion of inflammatory cytokine compared to normal tempera-ture and sub-hypothermia,which has important clinical significance.关键词
肠上皮细胞屏障/Caco-2 细胞株/中度低温/炎症因子Key words
intestinal epithelial cell barrier/Caco-2 cells/moderate hypothermia/inflammatory cytokine分类
医药卫生引用本文复制引用
刘先发,阳小灵,龚享铜,黄家淦,曾书琴,周丽程,张琼,张诗昊,陈侃,黄勤..中度低温对肠上皮细胞屏障氧化应激损伤后分泌炎症因子的影响及其临床意义[J].赣南医学院学报,2014,(4):498-501,4.基金项目
江西省卫生厅科技计划 （）
