赣南医学院学报Issue(6)：832-834,3.
姜黄素对缺氧复氧心肌细胞的保护作用及HSp70表达影响
protective Effect of Curcumin and HSp70 Expression on Hypoxia-Reoxygenation Cardiomyocyte Cells
摘要
Abstract
Objective:to explore the protection of curcumin on rat cardiac myocytes processed by hypoxia-reoxygenation and whether the protective effect is related to the expression of heat shock protein 70( HSp70 ). Methods:Rat cardiac myocytes were cultured primarily. MTT assay was applied to measure the IC50 of curcumin. Hypoxia-reoxygenation model was set up. The activity of lactate dehydrogenase( LDH)and the content of malondialdehyde( MDA)from each group were measured respectively. The apoptosis and necrosis of cells processed by hypoxia-reoxygenation were detected and the expression of HSp70 with immune histochemical method. Results:Compared with I/R group,the activity of LDH and the content of MDA of the concentrations of curcumin group decreased,but the expression of HSp70 increased. At the same time the rate of apoptosis and necrosis was reduced. Conclusions:Curcumin has protection and antiapoptotic effect on rat cardiac myocytes cultured in vitro and processed by hypoxia-reoxygenation. The protective effect is related to the in-creased expression of HSp70.关键词
姜黄素/缺氧复氧/心肌细胞/细胞凋亡/热休克蛋白70Key words
curcumin/hypoxia/reoxygenation/myocardial cells/apoptosis/heat shock protein 70分类
医药卫生引用本文复制引用
曾石秀,廖伟..姜黄素对缺氧复氧心肌细胞的保护作用及HSp70表达影响[J].赣南医学院学报,2014,(6):832-834,3.基金项目
江西省自然科学基金项目 （）
