感染、炎症、修复Issue(2)：70-75,6.DOI:10.3969/j.issn.1672-8521.2014.02.002
丙酮酸钠林格液减轻失血性休克大鼠脂质过氧化损伤及多脏器功能损害的研究
Study on pyruvate Ringer’s solution alleviates lipid peroxidative injury-induced multiple organ dysfunction during re-suscitation of hemorrhagic shock in rats
摘要
Abstract
Objective:To investigate the protective effects of pyruvate Ringer’s solution on lipid peroxidation in-jury-induced multiple organ dysfunction during resuscitation of hemorrhagic shock (HS)in rats. Methods:Hemor-rhagic shock was induced by withdrawing 45% of the calculated total blood volume within 20 minutes. Fifty-four SD rats were randomly divided into three groups (n=18). Group RN:HS with no fluid resuscitation. Group RL:HS resuscitated with commercial dl-lactate Ringer’s solution equal to 3 times the volume of shed blood. Group RP:HS resuscitated with pyruvate Ringer’s solution (pH 6.4)equal to 3 times the volume of shed blood. The mean arterial pressure (MAP)was monitored with PICCO cardiopulmonary capacity monitor,activities of Cu2+-Zn2+-superoxide dismutase (SOD)and the level of malondialdehyde (MDA)were determined 20 minutes before and 0,60,120,180,240,300,360 minutes after hemorrhage(T-20,T0,T60,T120,T180,T240,T300, T360).The content of MDA,oxidized and reduced glutathione,glutathione peroxidase,glutathione reductase and catalase in heart,lung,liver,kidney and j ej unum were determined at end of the experiment.Changes in creatine kinase (CK-MB),partial pressure of oxygen in artery (PaO2 ),alanine aminotransferase (ALT),diamine oxidase (DAO) and creatinine (Cr)were measured in arterial blood and plasma. The changes in water content in heart,lung,liv-er,kidney and jejunum were also measured.Results:At T120-360 after shock,the MAP in groups RL and RP was significantly higher than that of the group RN(P<0.05),and at T180-360,MAP in the group RP was signifi-cantly higher than that of the group RL(P<0.05). At T120-360,the blood Cu2+-Zn2+ SOD activities in group RP were significantly higher than that of group RN,but no significant difference was found when compared with those of the group RL. At T180-360,and the level of MDA was significantly lower in group RP than that in group RL(P<0.05). The content of MDA and oxidized glutathione in group RP was lower,but reduced glutathione was significantly higher than group RL in heart,lungs,liver and jejunum (P<0.05). The content of glutathione per-oxidase in group RP was lower but glutathione reductase was higher significantly than those in group RL in the heart,liver and jejunum (P<0.05). Protection of organ function and reduction of water content of tissues were pronounced in RP group as compared with RL group. Conclusions:Pyruvate Ringer’s solution may be significantly superior to lactate Ringer’s solution in alleviating multiple organ dysfunction and tissue edema induced by peroxida-tion after fluid resuscitation of HS.关键词
丙酮酸盐/休克失血性/抗氧化物酶/脂质过氧化物Key words
Pyruvate/Hemorrhagic shock/Antioxidant enzyme/Lipid peroxides引用本文复制引用
孟祥熙,刘先奇,方涛,童斌,白晓东,周方强,胡森..丙酮酸钠林格液减轻失血性休克大鼠脂质过氧化损伤及多脏器功能损害的研究[J].感染、炎症、修复,2014,(2):70-75,6.基金项目
全军医学科研“十一五”专项课题基金资助项目(06Z055) （06Z055）
