感染、炎症、修复Issue(3)：131-135,5.DOI:10.3969/j.issn.1672-8521.2014.03.001
热打击及中暑小鼠血清对小鼠肺微血管内皮细胞黏附单核细胞能力的影响
Effects of heat stress and the serum of mice suffering from heat stroke on adherent ability of monocytes to pulmonary microvascular endothelial cells in vitro
摘要
Abstract
Objective:To investigate the effect of heat stress and the serum of mice suffering from heat stroke on capacity of pulmonary microvascular endothelial cells (PMVECs)to express vascular cell adhesion molecule-1 (VCAM-1)and intercellular adhesion molecule-1 (ICAM-1),and adherent capacity of PMVECs to monocytes in vitro. Methods:PMVECs were isolated from neonatal mice by the method of secondary magnetic bead sorting. The morphology of PMVECs was observed under light microscope and identified by VE-Cadherin and Ⅷ factor stai-ning. PMVECs were randomly divided into 3 groups,including cells undergoing normal culture (37 ℃,as normal control),42 ℃ heat stress for 2 hours,and co-culture with serum of mice with heat stroke. Twenty-four hours later,total RNA was extracted,the quantitative PCR and Western blotting were used for the determination of mRNA and protein expressions of VCAM-1 and ICAM-1. The adherent capacity of PMVECs to monocytes was observed with fluorescence microscopy. Results:The PMVEC displayed typical"cobblestone"morphology under light microscopy,and they were positive with both VE-cadherin staining at cell-cell j unctions and Ⅷ factor,thus they were identified as endothelial cells. Compared to normal control group,the VCAM-1 and ICAM-1 mRNA and protein levels were decreased in heat stress group (P<0.05),but increased after stimulation of 10% serum of mice with heat stroke (P<0.01).Compared to the normal control group,the number of adherent THP-1 cells was similar in heat stress group,but the number of THP-1 cells was significantly increased in serum stimulation group,show-ing that the adherent capacity of PMVECs to monocyte was enhanced. Conclusions:Heat stress did not upregulate the ex-pression of endothelial cell adhesive molecules of PMVECs. But stimulation of serum of mice with heat stroke significantly up-regulate the expression of endothelial cell adhesion molecules,facilitating adhesion of monocytes,which produce or ag-gravate acute lung inj ury in heat stroke.关键词
热打击/肺微血管内皮细胞/单核细胞/黏附分子/中暑Key words
Heat sress/Pulmonary microvascular endothelial cell/Monocyte/Adhesion molecules/Heat stroke引用本文复制引用
刘亚楠,徐秋林,郭晓华,周耿标,王郑莲,陆杰富,邱俊明,苏磊..热打击及中暑小鼠血清对小鼠肺微血管内皮细胞黏附单核细胞能力的影响[J].感染、炎症、修复,2014,(3):131-135,5.基金项目
广东省自然科学基金博士启动项目(S2013040015661) （S2013040015661）
广东省医学科研基金资助项目(B2010176) （B2010176）
军队“十二五”医学科研基金重点项目(BWS12J018) （BWS12J018）
