甘肃农业科技Issue(1):31-33,3.DOI:10.3969/j.issn.1001-1463.2015.01.012
培养基对当归愈伤组织的诱导及分化影响研究
Study on the Effect of Medium on Induction and Differentiation of Callus of Angelica sinensis
摘要
Abstract
The seeds of Angelica sinensis are used to study the influence of different hormone combinations and media on callus induction and plant regeneration. The result shows that the optimal madium for callus induction is H+0.5 mg/L 2,4-D, and on it, the callus forming rate could reach to 97.92%;the optimal medium for induction of adventitious buds is 1/2 MS+0.5 mg/L 6-BA+1.0 mg/L NAA+0.5 mg/L ZT, and the highest induction rate is 90.3%;1/2 MS+0.5 mg/L IAA is the best for rooting, and the rooting rate could reach to 35.5%.关键词
当归/组织培养/再生体系/培养基Key words
Angelica sinensis/Tissue culture/Regeneration system/Medium分类
农业科技引用本文复制引用
王宏霞,王国祥,蔡子平..培养基对当归愈伤组织的诱导及分化影响研究[J].甘肃农业科技,2015,(1):31-33,3.基金项目
甘肃省农业科技创新专项(2011GAAS06-8、2012GAAS05-1、2013GAAS03-2、2013GAAS03-2);甘肃省农业生物技术研究与应用开发项目 ()