摘要
Abstract
Objective:To approach the practicability of the AllgloTM probe for the typing of IL-1 promoter . Methods: The genotyping for IL-1β promoter SNP was performed by the real-time Q-PCR with AllgloTM probe. We designed PCR primers and AllgloTM probe so as to detect the SNP site 3954C>T of IL-1β promoter,and optimized the concentration of primers and anneal temperature in accordance with PCR amplification efficiency and production specificity. Results: We successfully established the AllgloTM probe real-time Q-PCR detecting system, and in this two-step asymmetric PCR method, the primer density and the annealing temperature on the approximation PCR instrument were 0.4μM,58.9℃,respectively.We got the same genotyping by the system and sequenced in 100 healthy people detecting the site 3954C>T of IL-1β gene. Conclusion:The use of AllgloTM probe provides an attractive alternative for genotyping and detection of IL-1β gene SNP,which deserves to spread for its high-flux, low cost and convention.关键词
AllgloTM探针/白细胞介素-1/单核苷酸多态性Key words
AllgloTM probe/IL-1/SNP
