摘要
Abstract
Purpose To investigate the expression of the multidrug resistance and K-ras gene mutations in the colorectal cancer and their relationship. Methods:Using Immunohistochemistry and real-time quantitative PCR technique. Results:The expression rate of Pgp , Topo-II , CerbB-2 , GST-π and Ki-67 were 15 . 8%, 84 . 21%, 26 . 13%, 71 . 42%, 32 . 67% in the colorectal cancer . The expression rate of Pgp , Topo-II and K-ras mutation was not significant (P>0 . 05) with tumor incidence of gender , type , differentiation , depth of invasion and lymph node metastasis (P>0 . 05), while CerbB-2 , GST-π was significant with lymph node metastasis (P<0 . 05), Ki-67 was significant with tumor infiltration depth and lymph node metastasis (P<0 . 05). K-ras gene mutation rate was 28 . 94%, and was positively correlated with CerbB-2 expression (P<0 . 05). Conclusion:Combined detection of multidrug resistance- associated protein and K-ras gene mutations can contribute to judgment CRC sensitivity to chemotherapeutic drugs and provide the theory to formulate personalized chemotherapy .关键词
结直肠癌/K-ras基因突变/多药耐药/实时荧光定量PCRKey words
colorectal cancer/K-ras gene mutation/multidrug resistance/Real-time PCR
