广东药学院学报Issue(4)：399-403,5.DOI:10.3969/j.issn.1006-8783.2014.04.002
丹参脂溶性提取物隐丹参酮和丹参酮ⅡA 在大鼠体内的药动学研究
Study on pharmacokinetics of cryptotanshinone and tanshinone ⅡA from the liposoluble extract of Salvia miltiorrhiza in rats
摘要
Abstract
Objective To develop a reversed phase high-performance liquid chromatography for simultaneous determination cryptotanshinone and tanshinone ⅡA in rat plasma and to study on pharmacokinetics of the two tanshiones. Method The rats was administered the extract of Salvia miltiorrhiza ( 38. 4 mg · kg-1 cryptotanshinone and 70.1 mg·kg-1 tanshinone ⅡA), and the blood samples were collected from eye ophthalmic vein jungle venous plexus. After liquid liquid exatraction with acetonitrile-ethyl acetate, plasma concentrations of cryptotanshinone and tanshinone ⅡA were determined by HPLC. The 3P97 software package was used to calculate the pharmacokinetic parameters. Result A good linear relationship of cryptotanshinone was obtained from 0.05 to 5μg·mL-1 ,and the method recoveries were all from 99.7%-106.6%. A good linear relationship of tanshinone ⅡA was obtained from 0.02 to 5 μg·mL-1,and the method recoveries were all from 93.3%-102.5%. The pharmacokinetic parameters of cryptotanshinone and tanshinoneⅡA were as follows:t1/2(ka) was 0.924,0.993 h,t1/2(ke) was 2.632,2.126 h,Cmax was 0. 277,0.173μg·mL-1,AUC0-t was 1.855,1.034 μg·h·mL-1,CL/F(s) was 20.700,67.763 mL·h-1·mg-1. Conclusion The analysis method is rapid,simple and sensitive enough to be applied in the detection and pharmacokinetic study of cryptotanshinone and tanshinone ⅡA.关键词
丹参脂溶性提取物/隐丹参酮/丹参酮ⅡA/HPLC/药动学Key words
the liposoluble extract of Salvia miltiorrhiza/cryptotanshinone/tanshinone ⅡA/HPLC/pharmacokinetics分类
医药卫生引用本文复制引用
许丽媛,黄彩凤,黄鸿章,黎亮星,梁艳芬,郭波红,李宁..丹参脂溶性提取物隐丹参酮和丹参酮ⅡA 在大鼠体内的药动学研究[J].广东药学院学报,2014,(4):399-403,5.基金项目
国家自然科学基金项目(81173525) （81173525）
