广东药学院学报Issue(4)：417-421,5.DOI:10.3969/j.issn.1006-8783.2014.04.006
响应面法优化番石榴叶总黄酮的超声提取工艺
Optimization of ultrasonic assisted extraction of total flavonoids from leaves of Psidium guajava by response surface methodology
摘要
Abstract
Objective To study on the effects of different factors on the extraction of total flavonoids from leaves of Psidium guajava and optimize the ultrasound assisted extraction of total flavonoids by using response surface methodology. Methods Ethanol concentration, ratio of material to liquid and ultrasonic power were evaluated basing on single factor experiments with the yields of total flavonoids as index. Then response surface methodology with three-factors and three-levels was carried out for optimizing the extraction process of total flavonoids. Results The optimum conditions were as follows:extracting with 75%ethanol for twice and 30 minutes each time at a temperature of 50 ℃,liquid-solid ratio of 60∶1(mL∶g), and ultrasonic power of 150 W. Conclusion The actual yields of total flavonoids were very close to the predicted values,indicating that response surface analysis used in this experiment was feasible.关键词
响应面法(RSM)/番石榴叶/总黄酮/超声提取Key words
response surface methodology(RSM)/Psidium guajava/total flavonoids/ultrasonic extraction分类
医药卫生引用本文复制引用
谢郁峰,陈日佳,严春艳..响应面法优化番石榴叶总黄酮的超声提取工艺[J].广东药学院学报,2014,(4):417-421,5.基金项目
国家自然科学基金项目(81102779) （81102779）
广东药学院药科学院青年教师科研培育基金 （）
