广东药学院学报Issue(4)：439-443,5.DOI:10.3969/j.issn.1006-8783.2014.04.011
葛根芩连滴丸的质量标准研究
Study on quality standard of Gegen Qinlian dropping pills
摘要
Abstract
Objective To establish a method for the quality control of Gegen Qinlian dropping pills. Methods Puerariae lobatae Radix, Scutellariae Radix, Coptidis Rhizoma were identified by TLC and the contents of puerarin,berberine hydrochloride and baicalin were determined by HPLC. The mobile phase was consisted of methanol and 0.2% phosphoric acid( pH adjusted to 3.85 with triethylamine) at a flow rate of 1.0 mL·min-1 in a gradient elution. The detection wavelength was set at 265 nm and the column temperature was at 35 ℃. Results TLC identifications were performed with high resolution and without negative interference. The calibration curves were linear within the range of 0.817 5-13.08 μg·mL-1( r=0.999 9) for puerarin,5.160-82.56 μg·mL-1(r=0.999 9) for berberine hydrochloride and 12.39-198.2 μg·mL-1(r=0.999 8) for berberine,respectively. The average recoveries( n=9) were 101.6%( RSD=1.0%) for puerarin,98.2%( RSD=0.6%) for berberine hydrochloride and 100. 6%( RSD=1. 0%) for berberine, respectively. Conclusion The method was simple and accurate with a good reproducibility,and can be used for the quality control of Gegen Qinlian dropping pills.关键词
葛根芩连滴丸/葛根素/盐酸小檗碱/黄芩苷/高效液相色谱法Key words
Gegen Qinlian dropping pills/puerarin/berberine hydrochloride/baicalin/HPLC分类
医药卫生引用本文复制引用
宋凤兰,何洋,何琳,何立美..葛根芩连滴丸的质量标准研究[J].广东药学院学报,2014,(4):439-443,5.基金项目
2013年国家级大学生创新创业训练计划项目(201310573014) （201310573014）
