广东药学院学报Issue(4)：456-458,471,4.DOI:10.3969/j.issn.1006-8783.2014.04.015
毛细管电泳法同时测定三磷酸腺苷二钠注射液中苯甲醇与碳酸胍含量
Simultaneous determination of benzyl alcohol and guanidine carbonate in adenosine disodium triphosphate injection by HPCE
方海顺 1李芷如 2周远华 1苏广海1
作者信息
- 1. 广州市药品检验所，广东 广州510160
- 2. 广东药学院 药科学院，广东 广州510006
- 折叠
摘要
Abstract
Objective To establish a micellar electrokinetic capillary chromatography ( MEKC ) method to determine benzyl alcohol and guanidine carbonate simultaneously in adenosine disodium triphosphate injection. Methods Samples were analyzed on a uncoated capillary of 50 μm ×67 cm(50 cm of effective length) , and injected hydrodynamically ( 8 s, 3. 4 kPa ) . The applied voltage was 25. 0 kV and the temperature was kept at 25 ℃. Borate/SDS buffer(pH9.1) was used. The detection wavelength was 200 nm,and the running time was 15 min. Results The linear range of benzyl alcohol and guanidine carbonate were 0.037 5-1.688 mg/mL(r=0.996 9) and 0.040 48-2.024 mg/mL(r=0.999 4),respectively. The recoveries of benzyl alcohol and guanidine carbonate were 104. 7%-107. 0%( RSD=0. 9%, n=3 ) and 95. 7%-105.4%(RSD=3.2%,n=3),respectively. Conclusion The proposed HPCE method,with the advantages of high efficiency,sensitivity and reliability,could be applied in simultaneous determination of two accessories in adenosine disodium triphosphate injection.关键词
高效毛细管电泳/三磷酸腺苷二钠注射液/苯甲醇/碳酸胍Key words
HPCE/adenosine disodium triphosphate injection/benzyl alcohol/guanidine carbonate分类
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方海顺,李芷如,周远华,苏广海..毛细管电泳法同时测定三磷酸腺苷二钠注射液中苯甲醇与碳酸胍含量[J].广东药学院学报,2014,(4):456-458,471,4.
