广东药学院学报Issue(6)：683-687,5.DOI:10.3969/j.issn.1006-8783.2014.06.004
基于psbA-trnH序列对广藿香与藿香、广防风的分子鉴定
Molecular identification of Pogostemon cablin, Aagastache rugosus and Epimeredi indica based on psbA-trnH intergenic spacer sequences
摘要
Abstract
Objective To perform the molecular identification of Pogostemon cablin, Agastache rugosus and Epimeredi indica based on psbA-trnH intergenic spacer sequences. Methods Total DNA was extracted and psbA-trnH intergenic spacer sequences were amplified by PCR.PCR products were directly sequenced after purification.Sequences were analyzed with Clustal_X 1.8 and MEGA 5.05 software. Results The minimum inter-specific K2P distance was 0.159 2, higher than the maximum intra-specific K2P distance (0.004 3). The different samples from nine origin places of P. cablin were clustered into one clade in the Neighbor-Joining tree. Distinctive variation sites between P. cablin, A. rugosus and E. indica could be identified. Conclusion The psbA-trnH intergenic spacer sequences can be used to identificate P. cablin, A. rugosus and E. indica in molecule level.关键词
广藿香/藿香/广防风/psbA-trnH序列/鉴定Key words
Pogostemon cablin/Agastache rugosus/Epimeredi indica/psbA-trnH intergenic spacer/identification分类
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黄洁燕,卢慧娟,谢晖,姬生国..基于psbA-trnH序列对广藿香与藿香、广防风的分子鉴定[J].广东药学院学报,2014,(6):683-687,5.基金项目
广东省科技计划项目(2011B031700041) （2011B031700041）
