广东药学院学报Issue(6)：693-697,5.DOI:10.3969/j.issn.1006-8783.2014.06.006
AB-8型大孔树脂纯化东风桔总生物碱的工艺研究
Study on purifying the total alkaloids in Atalantia buxifolia with AB-8 macroporous resin
摘要
Abstract
Objective To study on purifying the total alkaloids in Atalantia buxifolia with macroporous resin. Methods The content of total alkaloids were determined by acid dye-colorimetry. The content of total alkaloids as index, the type of macroporous resin, sample volume, concentration, pH and volume of elution solvent were optimized by single-factor experiments. Results Optimal purification process conditions were as follows:optimal macroporous resin type AB-8, the concentration of sample solution 0.14 g·mL-1 with pH as 10.0, maximum sample volume 5 BV, washed by 9 BV purified water and 10 BV 95%ethanol, collected eluent of 95%ethanol. The average transfer rate of total alkaloids was 83.61%, and the adsorption and desorption rate were 89. 30% and 93. 64%, respectively. The RSD was 0. 30%. Conclusion The total alkaloids in Atalantia buxifolia can be purified effectively by AB-8 macroporous resin with good reproducibility.关键词
东风桔/总生物碱/大孔树脂/纯化/酸性染料比色法Key words
Atalantia buxifolia/total alkaloids/macroporous resin/purify/acid dye-colorimetry分类
医药卫生引用本文复制引用
谌江城,尹永芹,何宇愿,杜冠峰,宋国强,沈志滨..AB-8型大孔树脂纯化东风桔总生物碱的工艺研究[J].广东药学院学报,2014,(6):693-697,5.基金项目
科技部“十二五”重大新药创制(2011zx09102-007-03) （2011zx09102-007-03）
国家自然科学基金项目(81202885) （81202885）
广东省自然科学基金团队项目(10351022401000000) （10351022401000000）
