广东药学院学报Issue(6)：748-752,5.DOI:10.3969/j.issn.1006-8783.2014.06.019
基于1 H-NMR 法研究3种非甾体抗炎药对KLA诱导的RAW264.7细胞膜磷脂的变化
Effect of three non-steroidal anti-inflammatory drugs on the change of phospholipids in KLA-stimulated RAW264.7 cells by 1 H-NMR
摘要
Abstract
Objective To explore the relevance between inflammation and phospholipids in RAW264.7 cells by1 H-NMR. Methods RAW264. 7 cells were divided into five groups, including the blank group, the inflammation group, the diclofenac sodium group, the nimesulide group and the meloxicam group. The inflammatory stimulation was established by 100 ng · mL-1 of KLA treatment. 50 μg · mL-1 diclofenac sodium,45 μg · mL-1 nimesulide and 30 μg · mL-1 meloxicam were added into the different groups, respectively. The levels of TNF-αin culture supernatant were measured. The changes of phospholipids were detected by 1H-NMR spectra coupled with principal component analysis ( PCA). Results A separation tendency of phospholipids was observed in the blank group and three treatment groups when compared with the inflammation group.Conclusion The phospholipids in RAW264.7 cells may be changed before and after inflammatory stimulation.关键词
非甾体抗炎药/RAW264.7细胞/1 H-NMR法/主成分分析法/磷脂Key words
non-steroidal anti-inflammatory drugs/RAW264. 7 cells/1 H-NMR/principal component analysis/phospholipids分类
医药卫生引用本文复制引用
赵丽芳,彭海博,宋剑鳌,吴霞,冯毅凡..基于1 H-NMR 法研究3种非甾体抗炎药对KLA诱导的RAW264.7细胞膜磷脂的变化[J].广东药学院学报,2014,(6):748-752,5.基金项目
国家自然科学基金项目(21275036 （）
81202429) （）
