国际妇产科学杂志Issue(5)：509-512,4.
miR-200c过表达对OVCAR-3卵巢癌细胞生物学行为的影响
The Effect of miR-200c Over Expression on OVCAR-3 Epithelial Ovarian Cancer Biological Functions
摘要
Abstract
Objective: To investigate the effect of the miR-200c over-expression on the ability of proliferation, migration and invasion in OVCAR-3 ovarian cancer cells. Methods:Constructed retrovirus expression vector pMSCVpuro-miR-200c and transfected OVCAR-3 cells to overexpress miR-200c. By using CCK8 and Transwell methods to detect the ability of OVCAR-3 cells in proliferation and metastasis. Different expression genes involved in OVCAR-3 cells were detected by using gene chip before and after transfected with pMSCVpuro-miR-200c. Results:Compared with untransfected cells, the ability of proliferation, migration and invasion in pMSCVpuro-miR-200c transfected OVCAR-3 cell are significantly decreased (P<0.01). 190 up-regulated genes and 166 down-regulated genes were detected in OVCAR-3 cells after over-expressed miR-200c. Conclusions:As a tumor suppression gene,overexpression of miR-200c could inhibit the proliferation,migration and invasion of ovarian cancer cells.关键词
卵巢肿瘤/细胞增殖/细胞运动/肿瘤侵润/miR-200cKey words
Ovarian neoplasms/Cell proliferation/Cell movement/Neoplasm invasiveness/miR-200c引用本文复制引用
吴海湉,张越,林其德,张殊..miR-200c过表达对OVCAR-3卵巢癌细胞生物学行为的影响[J].国际妇产科学杂志,2014,(5):509-512,4.基金项目
国家自然科学基金（81172479）；上海市科学技术委员会科研计划项目 （）
