中国妇幼健康研究Issue(2)：271-272,286,3.DOI:10.3969/j.issn.1673-5293.2014.02.035
短时受精去除颗粒细胞对 IVF-ET治疗周期的影响
Influence of cumulus cells removal after short co-incubation of gametes on outcomes of in vitro fertilization and embryo transfer cycles
蒲艳 1丁洁 1顾斌 1郑爱燕 1李红 1王玮1
作者信息
- 1. 南京医科大学附属苏州市立医院生殖与遗传中心，江苏苏州215002
- 折叠
摘要
Abstract
Objective To analyze the influence of cumulus cells removal after short co-incubation of gametes on quality and outcomes of embryo in in vitro fertilization-embryo transfer (IVF-ET) treatment.Methods Data of infertility patients receiving IVF-ET treatment in the reproductive center of Suzhou Municipal Hospital Affiliated to Nanjing Medical University from January 2011 to January 2013 were retrospectively analyzed.According to whether second polar body was detected , 504 cycles were divided into two groups:group 1(detected group), group 2 (non-detected group).The data were compared between two groups .Results The MⅡoocytes rate (group 1:82.3%, group 2:85.9%), normal fertilization rate (group 1:55.6%, group 2:60.5%), and 1PN rate (group 1:3.2%, group 2:5.3%) between two groups were significantly different (allχ2 value was 0.000, all P<0.05).There were no significant differences in polyspermy rate, cleavage rate, good quality embryo rate, clinical pregnancy rate and implantation rate (all P>0.05).There were 32 cycles receiving early res-ICSI 6h after insemination due to fertilization failure and low fertilization rate in group 1, and 1 cycle received late res-ICSI 20h after insemination in group 2.Conclusion Mechanical removal of cumulus cells to detect the second polar body does not affect polyspermy rate, cleavage rate, good quality embryo rate , clinical pregnancy rate and implantation rate .The lower MⅡ oocytes rate, normal fertilization rate and 1PN rate may be caused by the early detection time .With second polar body detection , cancellof IVF can be avoided because of fertilization failure .It also decreases ICSI rate obviously in IVF treatment .Fertilization failure and low fertilization cases can be rescued by res-ICSI.关键词
第二极体观察/体外受精-胚胎移植/多精受精/补救性卵胞浆内单精子注射Key words
second polar body detection/in vitro fertilization-embryo transfer ( IVF-ET)/polyspermy/res-ICSI分类
医药卫生引用本文复制引用
蒲艳,丁洁,顾斌,郑爱燕,李红,王玮..短时受精去除颗粒细胞对 IVF-ET治疗周期的影响[J].中国妇幼健康研究,2014,(2):271-272,286,3.
