中国妇幼健康研究Issue(4)：603-605,3.DOI:10.3969/j.issn.1673-5293.2014.04.019
哮喘儿童诱导痰中炎性细胞及细胞因子的检测
Detection of inflammatory cells and cytokine in induced sputum of children with asthma
摘要
Abstract
Objective To understand the effect and mechanism of inflammatory cells and cytokine in childhood asthma .Methods Experimental research strategy was adopted to select 30 cases at acute stage and at clinical remission stage , and 30 normal controls as well . Their pulmonary function was tested and sputum induction method was used to calculate sputum inflammatory cells .The concentrations of sputum IL-8 and IL-17 were measured by enzyme-linked immunosorbent assay .Single factor variance analysis was conducted to compare mean values of three groups , SNK method was used to make pair comparison , and linear correlation analysis was carried out .Results Single factor variance analysis showed that the contents of neutrophils , IL-8 and IL-17 in induced sputum at acute stage group were higher than clinical remission group and control group , and the differences were significant (F value was 172.16, 135.69 and 24.28, respectively, all P<0.05).In clinical remission stage group the contents of neutrophils , IL-8 and IL-17 in induced sputum were significantly higher than the normal control group (F value was 67.80, 54.64 and 17.36, respectively, all P<0.05).At acute stage and clinical remission stage the contents of IL-8 and IL-17 were positively correlated with neutrophils (r>0, both P<0.05).Conclusion All of neutrophils, IL-8 and IL-17 involve in the pathogenesis of asthma , which provides basis for further exploration on diagnosis and treatment .关键词
支气管哮喘/儿童/诱导痰/气道炎性细胞/白介素-8/白介素-17Key words
bronchial asthma/children/induced sputum/airway inflammatory cells/interleukin-8 (IL-8)/interleukin-17 (IL-17)分类
医药卫生引用本文复制引用
张春霞,任少敏,张晓蒙,王继春..哮喘儿童诱导痰中炎性细胞及细胞因子的检测[J].中国妇幼健康研究,2014,(4):603-605,3.基金项目
2012年内蒙古自然科学基金资助项目 （）
