摘要
Abstract
Objective To investigate the effect of matrine combined with cisplatin on inhibiting human cervical cancer SiHa cells and on tumor suppressor in lung cancer 1 (TSLC1) gene expression.Methods Logarithmic growth phase of SiHa cells were divided into control group, matrine group, cisplatin group and combination group.Inhibition rate was measured with MTT method.The change of TSLC1 gene mRNA expression was detected by RT-PCR process in each group.Results Inhibition rates of SiHa cells in the control group, matrine group, cisplatin group and the combination group were 0, 61.24%, 70.31%and 74.20%, respectively.The inhibition rates of medicine intervention groups were higher than the control group (t value was 14.21, 17.44 and 11.20, respectively, all P<0.05), and the combination group was higher than cisplatin and matrine group (t value was 4.47 and 3.21, respectively, both P<0.05).There was no significant difference between cisplatin group and matrine group (t=0.71, P>0.05).The levels of TSLC1 mRNA expression after the intervention on SiHa cells in the control group, matrine group, cisplatin group and the combination group were 1.00 ±0.00μg/mL, 23.53 ±0.01μg/mL, 84.33 ±0.03μg/mL and 90.23 ±0.01μg/mL, respectively.Those of the medicine intervention groups were significantly higher than the control group (z was 9.41, 10.72 and 13.23, respectively, all P<0.05).There was no significant difference between matrine group and the combination group in TSLC1 mRNA expression level (z=1.24,P>0.05), but both of them were higher than cisplatin group (z value was 6.04 and 5.47, respectively, both P<0.05).Factorial design analysis of variance showed that cisplatin and matrine were influencing factors of inhibiting SiHa cells and TSLC1 gene expression.There was an interaction effect between them so that it generated increasing effect by combination.Conclusion Matrine combined with cisplatin can inhibit the proliferation of human cervical cancer SiHa cells and improve TSLC1 mRNA expression level.关键词
宫颈癌/SiHa细胞/苦参碱/顺铂/肺癌肿瘤抑制因子1Key words
cervical cancer/SiHa cells/cisplatin/matrine/tumor suppressor in lung cancer-1(TSLC1)分类
医药卫生
