黄燕萍 1王祥 2高娜 3赵文静 4孙艳 4马丹萍 1刘丽1
作者信息
- 1. 西安交通大学第一附属医院儿科，陕西 西安 710061
- 2. 莱州市人民医院儿科，山东 莱州 261400
- 3. 陕西省人民医院，陕西 西安 710068
- 4. 西安市中心医院，陕西 西安 710003
- 折叠
摘要
Abstract
Objective To study the influence of diallyl trisulfide on IgA nephropathy renal interstitial fibrosis and to explore its possible renal protective mechanism. Methods IgA nephropathy fibrotic model in rats were established in study by combining IgA nephropathy model with unilateral ureteral obstruction. The healthy SD rats were randomly and equally divided into 4 groups: normal control group, model control group, high-dose dially trisulfide ( DATS) treatment group and low-dose DATS treatment group. The above groups were lavaged with corresponding drug. The urine and blood of rats were examined for urinary red blood cell count, blood urea nitrogen, TNF-αand α-SMA before intervention, 14 and 28 days after intervention. At the 28th day, all rats were sacrificed and their pathomorphological changes in kidneys were observed by immune fluorescence microscope. Results Compared with model control group, the levels of urine erythrocyte (model control group —high-dose DATS treatment group, at 14/28 days after intervention,t =1. 12/5. 58; model control group— low-dose DATS treatment group, at 14/28 days after intervention, t=1. 62/7. 51), blood urea nitrogen, TNF-α (model control group—high-dose DATS treatment group, at 14/28 days after intervention, t =3. 48/11. 95; model control group —low-dose DATS treatment group, at 14/28 days after intervention,t=4. 76/12. 78) andα-SMA (model control group—low-dose DATS treatment group, at 14/28 days after intervention,t=5. 38/5. 77) reduced in both DATS treatment groups. The degree of IgA deposits at glomerular mesangial area and the renal pathological fibrosis relieved in both DATS treatment groups. There were significant differences between high-dose and low-dose DATS treatment groups in reducing the levels of urine erythrocyte (at 28 days after intervention,t=1. 99), TNF-α (at 28 days after intervention,t=3. 73) and α-SMA (at 14/28 days after intervention,t=5. 60/4. 81) (all P<0. 05). The effect of low-dose DATS treatment was more obvious. Conclusion The levels of TNF-α and α-SMA increase progressively in IgA nephropathy fibrosis developing. The dially trisulfide can relieve the clinical symptoms and pathological damages in IgA nephropathy fibrotic rats, and can prevent the occurrence of renal interstitial fibrosis by reducing the levels of serum TNF-α and α-SMA.关键词
IgA肾病/肾间质纤维化/肿瘤坏死因子-α/α-平滑肌肌动蛋白Key words
IgA nephropathy ( IgAN)/renal interstitial fibrosis ( RIF)/tumor necrosis factor-α( TNF-α)/alpha smooth muscle actin (α-SMA)分类
医药卫生
