医学分子生物学杂志Issue(3)：125-131,7.DOI:10.3870/j.issna.1672-8009.2014.03.001
miR-1273 g-3 p通过靶向CNR1调节A549细胞迁移能力
MiR-1273g-3p Regulates A549 Cell Migration by Targeting CNR1
摘要
Abstract
Objective To identify the target gene of hsa-miR-1273g-3p and investigate its bio-logical function in human A549 cells.Methods The expression level of miR-1273g-3p in A549, H460 and H1299 cells was detected by quantitative real-time PCR ( qRT-PCR) .The target gene of miR-1273g-3p was predicted by bioinformatics and confirmed by qRT-PCR, Western blotting and luciferase report assay .The wound-healing assay and MTS assay were used to examine the biological function of miR-1273g-3p.Results miR-1273g-3p was over-expressed in A549, H460 and H1299 cells.qRT-PCR, Western blotting and luciferase report assay confirmed that miR-1273g-3p could target CNR1 and it could regulate CNR1 at mRNA and protein level.MiR-1273g-3p could promote the migration of A549 cells by inhibiting the expression of CNR 1.Its inhibitory effect on the prolifer-ation of A549 cells was not significant.Conclusion CNR1 is a target gene of miR-1273g-3p.MiR-1273g-3p can promote A549 cell migration by targeting CNR1.关键词
miR-1273g-3p/CNR1/细胞迁移Key words
miR-1273g-3p/CNR1/cell migration分类
医药卫生引用本文复制引用
侯绿滨,邵宁生,苏雪婷,秦性良,丁红梅,黄皑雪,李慧,葛兴枫,赵强,李洁..miR-1273 g-3 p通过靶向CNR1调节A549细胞迁移能力[J].医学分子生物学杂志,2014,(3):125-131,7.基金项目
国家重点基础研究发展计划（973计划）（ No．2011 CB811300，2010 CB912801），国家自然科学基金（No．31100569，30971630，31370794）This work was supported by grants from the Special Funds for Major State Basic Research Program of China （973 Program）（No．2011CB811300，2010 CB912801） and the National Natural Science Foundation of China （）
