医学分子生物学杂志Issue(3)：175-178,4.DOI:10.3870/j.issna.1672-8009.2014.03.010
C／EBPβ在星型胶质细胞中对TGF-β／Smad3通路的调控及分子机制研究
Regulatory Effect of C/EBPβon the TGF-β/Smad3 Pathway in As-trocytes
摘要
Abstract
Objective To examine the regulatory effect of C/EBPβon the TGF-β/Smad3 path-way in astrocytes and the molecular mechanism .Methods Real-time PCR and Western blotting were used to examine the effect of up-regulated expressions of C/EBPβand Smad3 on TGF-βin as-trocytes.The proliferation rate and the apoptosis rate of astrocytes were detected after up-regulation or silencing of the C/EBPβgene.Results The expression levels of TGF-βand Smad3 were obviously increased in astrocytes overexpressing C/EBPβ, while the expression level of TGF-βwas decreased in astrocytes overexpressing Smad 3.The proliferation rate was significantly elevated and the apoptosis rate declined in astrocytes with C/EBPβgene up-regulated [ ( 73.26 ±4.57 )%vs. ( 138.29 ± 3.36)%] for proliferation rate; [ (24.69 ±2.06)%vs.(6.32 ±1.63)%] for apoptosis rate, P<0.01) .In astrocytes with silencing of C/EBPβ, the proliferation rate [ (73.26 ±4.57)%vs. (97.54 ±3.72)%] was significantly declined and the apoptosis rate elevated [ (24.69 ±2.06)%vs.(14.76 ±1.43)%, P<0.01] .Conclusion C/EBPβcan activate the TGF-β/Smad3 path-way in astrocytes and promote the proliferation of astrocytes by inducing the expression of TGF-β.关键词
C/EBPβ/TGF-β/Smad3通路/星型胶质细胞/交通性脑积水Key words
C/EBPβ/TGF-β/Smad3 pathway/astrocytes/communicating hydrocephalus分类
医药卫生引用本文复制引用
陈一鸣,黄承光..C／EBPβ在星型胶质细胞中对TGF-β／Smad3通路的调控及分子机制研究[J].医学分子生物学杂志,2014,(3):175-178,4.基金项目
国家自然科学基金（No．81171130）This work was supported by a grant from the National Natural Science Foundation of China （）
