医学分子生物学杂志Issue(6)：318-321,4.DOI:10.3870/j.issn.1672-8009.2014.06.002
牛蒡苷元通过调节 MMP-2，MMP-9表达抑制增生性瘢痕的形成
Arctigenin Preventsthe Formation of Hypertrophic Scars by Reducing the Expression of MMP-2 and MMP-9
摘要
Abstract
Objective To investigate the effects of arctigenin on the formation of hypertrophic scars and the possible mechanism. Methods Twenty-five rabbits were randomly divided into five groups: control group, model group, 0. 5 mg / mL arctigenin group, 2 mg / mL arctigenin group and 6 mg / mL arctigenin group. Hypertrophic scars were induced on the ventral surface of rabbit ears and treated with arctigenin at different doses. The wound healing and hyperplasia of the scars were ob-served. The scar tissues were removed for histopathological detection with HE staining, Sirius red staining and Masson staining and for detection of expression of MMP-2 and MMP-9 with Western blotting 6 weeks after treatment. Results HE staining, Sirius red staining and Masson staining showed that arctigenin (2 mg / mL) could significantly inhibit the hyperplasia of the scars. The scars were flatter, the number of fibroblasts and the density of collagen were less in the arctigenin-treated groups than in the model group. Western blotting showed that the expression levels of MMP-2 and MMP-9 were significantly decreased in 2 mg / mL arctigenin group. Conclusion Arctigenin may prevent the formation of hypertrophic scars by reducing the expression of MMP-2, MMP-9 and it may be used to treat hyperplastic scars.关键词
牛蒡苷元/增生性瘢痕/基质金属蛋白酶Key words
arctigenin/hypertrophic scar/matrix metalloproteinases分类
医药卫生引用本文复制引用
杜志超,王姗,卢兹凡,王玉琨,汪莉..牛蒡苷元通过调节 MMP-2，MMP-9表达抑制增生性瘢痕的形成[J].医学分子生物学杂志,2014,(6):318-321,4.基金项目
国家自然科学基金(No.81102259)This work was supported by a grant from the National Natural Science Foundation of China (No.81102259) （No.81102259）
